Charostad Javad, Navidfar Tahere, Kiani Masoumeh, Schinitzler Paul, Astani Akram
Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Basic Sciences, Shoushtar Faculty of Medical Sciences, Shushtar University of Medical and Science, Shushtar, Iran.
Iran J Microbiol. 2024 Dec;16(6):786-791. doi: 10.18502/ijm.v16i6.17257.
Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir.
In our experimental study, was dissolved in DMSO, then diluted in DMEM medium. Acyclovir was used at a concentration of 100 μM in all procedures. The antiherpetic activity and Antiviral activity of were evaluated in Vero cells (African green monkey kidney cells) by using the plaque reduction assay.
Inhibitory concentrations of 50% (IC) of for HSV-1 and HSV-2 were determined at 0.00025% and 0.00015%, respectively. was introduced at various stages of viral infection and significantly inhibited HSV-1 and HSV-2 infectivity by > 95.5% and 89%, respectively, when virus was pre-treated before addition to the cells. No HSV-1 or HSV-2 activity was detected in cells treated prior to and following viral infection.
These results indicate that demonstrates antiherpetic activity in the early phase of viral infection and could be used as potential antiviral agent.
药用植物是许多传染性和非传染性疾病的主要治疗方法。在本研究中，我们评估了[具体药物名称未给出]对单纯疱疹病毒1型和2型的抗病毒活性，并将其与抗病毒药物阿昔洛韦进行比较。
在我们的实验研究中，[具体药物名称未给出]溶解于二甲基亚砜（DMSO）中，然后在杜氏改良 Eagle 培养基（DMEM）中稀释。在所有实验步骤中，阿昔洛韦的使用浓度均为100μM。通过蚀斑减少试验在非洲绿猴肾细胞（Vero细胞）中评估[具体药物名称未给出]的抗疱疹活性和抗病毒活性。
[具体药物名称未给出]对单纯疱疹病毒1型和2型的50%抑制浓度（IC50）分别测定为0.00025%和0.00015%。在病毒感染的各个阶段加入[具体药物名称未给出]，当在病毒加入细胞之前进行预处理时，[具体药物名称未给出]分别显著抑制单纯疱疹病毒1型和2型的感染性，抑制率分别>95.5%和89%。在病毒感染之前和之后处理的细胞中未检测到单纯疱疹病毒1型或2型的活性。
这些结果表明，[具体药物名称未给出]在病毒感染的早期阶段表现出抗疱疹活性，可作为潜在的抗病毒药物。
