Zarei Moein, Żwir Marek J, Michalkiewicz Beata, Gorący Jarosław, El Fray Miroslawa
Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, Poland.
Department of Catalytic and Sorbent Materials Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, Poland.
J Biomed Mater Res B Appl Biomater. 2025 Jan;113(1):e35525. doi: 10.1002/jbm.b.35525.
Fabricating complex hierarchical structures mimicking natural vessels and arteries is pivotal for addressing problems of cardiovascular diseases. Various fabrication strategies have been explored to achieve this goal, each contributing unique advantages and challenges to the development of functional vascular grafts. In this study, a three-layered tubular structure resembling vascular grafts was fabricated using biocompatible and biodegradable copolymers of poly(butylene succinate) (PBS) using advanced manufacturing techniques. The outer layer was fabricated by template-assisted electrospinning utilizing a 3D-printed scaffold with a precise hexagonal pore design as the template, and the inner layer was coated with gelatin through perfusion. Cellulose nanocrystals (CNCs) were incorporated into electrospun fibers to enhance mechanical properties. The gelatin coating was applied to the lumen using perfusion coating, resembling the inner layer. Integration of 3D-printed structures with electrospun fibers via template-assisted electrospinning and gelatin coating resulted in a seamless multilayered scaffold. Mechanical testing demonstrated robustness, surpassing natural arteries in some aspects, while the gelatin coating significantly reduced liquid leakage, ensuring leak-free functionality. Cytotoxicity assessment confirmed the biocompatibility of processed materials with fibroblast cells, supporting potential for medical applications.
制造模仿天然血管和动脉的复杂分层结构对于解决心血管疾病问题至关重要。为实现这一目标，人们探索了各种制造策略，每种策略都为功能性血管移植物的发展带来了独特的优势和挑战。在本研究中，使用先进制造技术，利用聚丁二酸丁二醇酯（PBS）的生物相容性和可生物降解共聚物制造了一种类似于血管移植物的三层管状结构。外层通过模板辅助静电纺丝制造，使用具有精确六边形孔设计的3D打印支架作为模板，内层通过灌注涂覆明胶。将纤维素纳米晶体（CNCs）掺入静电纺丝纤维中以增强机械性能。明胶涂层通过灌注涂层应用于管腔，类似于内层。通过模板辅助静电纺丝和明胶涂层将3D打印结构与静电纺丝纤维整合在一起，形成了无缝的多层支架。机械测试表明其具有坚固性，在某些方面超过了天然动脉，而明胶涂层显著减少了液体泄漏，确保了无泄漏功能。细胞毒性评估证实了加工材料与成纤维细胞的生物相容性，支持了其在医学应用中的潜力。
