Zhou Zhixin, Han Bing, Wang Yu, Lin Nina, Zhou Zhongqiu, Zhang Yuan, Bai Ying, Shen Ling, Shen Yanfei, Zhang Yuanjian, Yao Honghong
School of Chemistry and Chemical Engineering, Jiangsu Provincial Key Laboratory of Critical Care Medicine, Southeast University, Nanjing, China.
Department of Pharmacology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University, Nanjing, China.
Nat Commun. 2024 Dec 30;15(1):10900. doi: 10.1038/s41467-024-55364-x.
While circular RNAs (circRNAs) exhibit lower abundance compared to corresponding linear RNAs, they demonstrate potent biological functions. Nevertheless, challenges arise from the low concentration and distinctive structural features of circRNAs, rendering existing methods operationally intricate and less sensitive. Here, we engineer an intelligent tetrahedral DNA framework (TDF) possessing precise spatial pattern-recognition properties with exceptional sensing speed and sensitivity for circRNAs. The signal output of TDF sensor occurs only when multivalent spatial pattern-recognition of a circRNA in unamplified samples. Using this sensor, we visualize the real-time response of endogenous circRNA expression in vitro neuronal cells and in vivo brain between pre-stroke and post-stroke male mice, identify the patients with acute ischemic stroke in clinical samples, as well as track the delivery of circRNA in photochromic stroked animal model. Thus, the TDF sensor provides a fast and sensitive tool for the detection of circRNA abundance in both physiological and pathophysiological conditions.
虽然环状RNA（circRNA）与相应的线性RNA相比丰度较低，但它们具有强大的生物学功能。然而，circRNA的低浓度和独特的结构特征带来了挑战，使得现有方法操作复杂且灵敏度较低。在这里，我们设计了一种智能四面体DNA框架（TDF），它具有精确的空间模式识别特性，对circRNA具有出色的传感速度和灵敏度。TDF传感器的信号输出仅在未扩增样品中circRNA的多价空间模式识别时才会发生。使用该传感器，我们可视化了体外神经元细胞和体内雄性小鼠中风前和中风后脑内源性circRNA表达的实时反应，在临床样本中识别急性缺血性中风患者，并在光致变色中风动物模型中追踪circRNA的递送。因此，TDF传感器为在生理和病理生理条件下检测circRNA丰度提供了一种快速且灵敏的工具。
