Espinoza Nallely, Spinard Edward, Rai Ayushi, Ramirez-Medina Elizabeth, Valladares Alyssa, Meyers Amanda, Borca Manuel V, Gladue Douglas P
Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, 11944, USA.
National Bio and Agro-Defense Facility, Agricultural Research Service, USDA, Manhattan, KS, 66506, USA.
Sci Rep. 2024 Dec 30;14(1):31613. doi: 10.1038/s41598-024-80170-2.
For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on culling infected farms, and strict biosecurity procedures. Recently a vaccine, ASFV-G-ΔI177L was approved for use in Vietnam. In this study we evaluate the long-term stability of lyophilized ASFV-G-ΔI177L. Understanding the stability of different formulations of vaccines is information necessary for deployment of vaccines to ASF outbreak areas, particularly those that do not have a reliable well established cold chain to ensure conservation of vaccine quality. In this report, we determined that ASFV-G-ΔI177L, when lyophilized under specific conditions, is stable for up to one year at 4 °C, with similar vaccine titers after storage. Next-generation sequencing analysis also determined that lyophilization and long-term storage under these conditions had no effect on the genome of ASFV as the genome remained genetically identical to the original non-lyophilized form.
一个多世纪以来，非洲猪瘟（ASF）一直引发疫情，给养猪业造成毁灭性损失。当前的ASF大流行没有停止的迹象，仍在继续蔓延，导致更多国家出现疫情。目前的防控主要依靠扑杀受感染的养殖场，以及严格的生物安全程序。最近，一种名为ASFV-G-ΔI177L的疫苗在越南获批使用。在本研究中，我们评估了冻干的ASFV-G-ΔI177L的长期稳定性。了解不同疫苗制剂的稳定性是将疫苗部署到ASF疫情地区所必需的信息，特别是那些没有可靠且完善的冷链来确保疫苗质量保存的地区。在本报告中，我们确定，在特定条件下冻干的ASFV-G-ΔI177L在4℃下可稳定保存长达一年，储存后疫苗效价相似。下一代测序分析还确定，在这些条件下进行冻干和长期储存对ASFV的基因组没有影响，因为基因组与原始的非冻干形式在基因上保持一致。
