Honkpehedji Yabo J, Kildemoes Anna O, Stam Koen A, Nguyen Dieu L, Veldhuizen Tom, van Diepen Angela, Esen Meral, Kremsner Peter G, Wuhrer Manfred, Adegnika Ayôla A, Hokke Cornelis H, Yazdanbakhsh Maria
Centre de Recherche Médicales de Lambaréné (CERMEL), Lambaréné, Gabon.
Leiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center (LUMC), Albinusdreef 2, 2333ZA, Leiden, Zuid-Holland, The Netherlands.
Sci Rep. 2024 Dec 30;14(1):31879. doi: 10.1038/s41598-024-83366-8.
Antibody glycosylation patterns can affect antibody functionality and thereby contribute to protection against invading pathogens. During pregnancy, maternal antibodies can be transferred through the placenta and contribute to modulating both the mother's and her child's immune responses. Although several studies of IgG glycosylation during pregnancy have been carried out, very few cohorts studied were from sub-Saharan Africa, where exposure to microorganisms and parasites is high. In Lambaréné, Gabon, 106 pregnant women in their third trimester were enrolled into this study. At enrolment, urine, stool, and blood samples were collected from the mothers to assess Schistosoma haematobium (S. haematobium), Plasmodium falciparum (P. falciparum) and other parasite infections. During delivery, cord blood samples were collected. The children were followed, and blood samples were collected at 9 and 12 months of age. IgG Fc glycosylation was measured by liquid chromatography-mass spectrometry, determining fucosylation, galactosylation, sialylation, bisection, and sialylation per galactose (SA/gal). Among the 106 pregnant women, 33 (31%) were infected by at least one parasite. The antibody glycosylation patterns in maternal and cord blood showed distinct profiles when compared to that of infants at 9 and 12 months. IgG galactosylation was higher in maternal/cord blood, while fucosylated IgG was higher in children up to 1 year of age. Maternal parasitic infection was associated with lower IgG2 and IgG3/IgG4 galactosylation in cord blood and lower IgG3/IgG4 galactosylation in children. When maternal IgG galactosylation and, consequently, cord blood were categorized as high, children at 9 and 12 months of age showed higher IgG galactosylation compared to children of mothers with low IgG galactosylation. As IgG Fc galactosylation can have functional consequences, it might provide valuable information for developing effective preventive and treatment strategies for vulnerable populations.
抗体糖基化模式可影响抗体功能，从而有助于抵御入侵病原体。在孕期，母体抗体可通过胎盘转移，有助于调节母亲及其孩子的免疫反应。尽管已开展了多项关于孕期IgG糖基化的研究，但很少有研究队列来自撒哈拉以南非洲地区，该地区微生物和寄生虫暴露率很高。在加蓬的兰巴雷内，106名孕晚期孕妇参与了本研究。入组时，采集母亲的尿液、粪便和血液样本，以评估埃及血吸虫、恶性疟原虫和其他寄生虫感染情况。分娩时，采集脐带血样本。对儿童进行随访，并在其9个月和12个月大时采集血液样本。通过液相色谱-质谱法测量IgG Fc糖基化，测定岩藻糖基化、半乳糖基化、唾液酸化、二分法以及每半乳糖的唾液酸化程度（SA/gal）。在这106名孕妇中，33名（31%）至少感染了一种寄生虫。与9个月和12个月大婴儿的抗体糖基化模式相比，母体和脐带血中的抗体糖基化模式呈现出不同的特征。母体/脐带血中的IgG半乳糖基化水平较高，而1岁以下儿童中岩藻糖基化IgG水平较高。母体寄生虫感染与脐带血中较低的IgG2以及IgG3/IgG4半乳糖基化和儿童中较低的IgG3/IgG4半乳糖基化有关。当母体IgG半乳糖基化以及相应的脐带血被分类为高水平时，9个月和12个月大儿童的IgG半乳糖基化水平高于母体IgG半乳糖基化水平低的母亲所生的儿童。由于IgG Fc半乳糖基化可能具有功能影响，它可能为为脆弱人群制定有效的预防和治疗策略提供有价值的信息。
