Tena-Tomás Cristina, Bouyou-Akotet Marielle K, Kendjo Eric, Kombila Maryvonne, Kremsner Peter G, Kun Jürgen F J
Department of Parasitology, Institute for Tropical Medicine, Wilhelmstr. 27, 72074 Tübingen, Germany.
Parasitol Res. 2007 Sep;101(4):1045-50. doi: 10.1007/s00436-007-0585-9. Epub 2007 May 29.
In areas where malaria transmission is stable, infants are often born to mothers who had Plasmodium falciparum infections during pregnancy. A significant number become exposed to infected erythrocytes or soluble parasite products with subsequent fetal immune priming or tolerance in utero. We performed ELISA to asses IgG and IgM seropositivity rates against three PfEMP1 DBL-alpha domains from 42 maternal-cord paired samples obtained at delivery from a hyperendemic area in Gabon. IgG was present in up to 80% of the cord serum samples, while IgM was found in only 20% of the same samples. These levels were not dependent on the parity of the mother or the peripheral and placental infectious status. The presence of IgM against DBL-alpha domain in cord serum samples suggests that this component is able to cross the placental barrier and mount a fetal immune response.
在疟疾传播稳定的地区，婴儿通常出生于孕期感染恶性疟原虫的母亲。大量婴儿会接触到受感染的红细胞或可溶性寄生虫产物，随后在子宫内引发胎儿免疫致敏或耐受。我们进行了酶联免疫吸附测定（ELISA），以评估来自加蓬一个高度流行地区分娩时采集的42对母婴配对样本针对三种恶性疟原虫红细胞膜蛋白1（PfEMP1）双结构域α（DBL-α）的IgG和IgM血清阳性率。高达80%的脐带血清样本中存在IgG，而在相同样本中仅20%发现IgM。这些水平不取决于母亲的胎次以及外周和胎盘的感染状况。脐带血清样本中存在针对DBL-α结构域的IgM表明该成分能够穿过胎盘屏障并引发胎儿免疫反应。
