Can Venhar Gurbuz, Gunay Ozlem Cesur, Cesur Sinem
Department of Medical Biology, Karabuk University Faculty of Medicine, Karabük, 78050, Turkey.
Carsamba State Hospital, Samsun, 55500, Turkey.
Sci Rep. 2024 Dec 30;14(1):31731. doi: 10.1038/s41598-024-82773-1.
Type 2 diabetes (T2DM) is a group of chronic and systemic metabolic diseases. Nephropathy (NP) and neuropathy (NR) are two common complications that severely affect the quality of life of patients with T2DM. In this study, our aim was to investigate the association between TNF-alpha (-308) gene polymorphism and the risk of developing NP and NR in patients with T2DM We also aimed to determine the association between TNF-alpha (-308) gene polymorphism and several demographic characteristics and biochemical parameters of patients with T2DM developed NP and/or NR. First, blood samples of patients were collected, and DNA isolation was performed. Then, real-time PCR analysis was used for genotyping the TNF-α (-308) gene utilising fluorescently labelled probes. Patients' demographical data and biochemical parameters were also obtained. Our study did not detect any significant difference in the frequency of possessing the gene polymorphism (A allele) between the groups. Additionally, no significant difference was detected when the data on various biochemical parameters were evaluated in GG, GA, and AA genotypes.
2型糖尿病（T2DM）是一组慢性全身性代谢疾病。肾病（NP）和神经病变（NR）是两种常见并发症，严重影响T2DM患者的生活质量。在本研究中，我们的目的是调查肿瘤坏死因子-α（TNF-α）（-308）基因多态性与T2DM患者发生NP和NR风险之间的关联。我们还旨在确定TNF-α（-308）基因多态性与发生NP和/或NR的T2DM患者的一些人口统计学特征和生化参数之间的关联。首先，采集患者的血液样本并进行DNA分离。然后，使用实时PCR分析，利用荧光标记探针检测TNF-α（-308）基因的基因型。还获取了患者的人口统计学数据和生化参数。我们的研究未检测到两组之间拥有基因多态性（A等位基因）频率的任何显著差异。此外，在GG、GA和AA基因型中评估各种生化参数数据时，未检测到显著差异。
