Vincent Richard, Rapoport David, Balchandani Priti, Borrello Joseph, Schotsaert Michael, Karlicek Robert, Laghlali Gabriel, Warang Prajakta, Park Seokchan, Singh Gagandeep, Morgan Isabella, Paredes James, Rathnasinghe Raveen, Wolf Jacob, García-Sastre Adolfo
Division of General Internal Medicine, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Sci Rep. 2024 Dec 30;14(1):31799. doi: 10.1038/s41598-024-82901-x.
Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototype portable UV-C device to sterilize high flows of viral-contaminated air from a simulated patient source at airflow rates of up to 100 l/m. Our device consisted of a central quartz tube surrounded 6 high-output UV-C lamps, within a larger cylinder allowing recirculation past the UV-C lamps a second time before exiting the device. Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. Turning on varying numbers of lamps controlled the dose of UVC. Viability experiments at low, medium and high (100 l/min) flows of contaminated gas were conducted with 6, 4, 2 and 1 lamp activated (single-pass and recirculation were tested). Our data show 5-log reduction in plaque forming units from a single lamp (single- pass and recirculated conditions) at high and low flows. UVC dose at 100 l/m was calculated at 11.6 mJ/cm single pass and 104 mJ/cm recirculated. The protype device shows high efficacy in killing nebulized influenza virus in a high flow of contaminated air.
包括无创通气、持续气道正压通气和高流量鼻导管给氧在内的呼吸干预措施会产生感染性气溶胶，可能增加空气传播疾病（严重急性呼吸综合征冠状病毒2、流感病毒）传播给医护人员的风险。我们研发并测试了一种便携式紫外线C（UV-C）原型设备，以对来自模拟患者源的高流量病毒污染空气进行消毒，气流速度高达100升/分钟。我们的设备由一根中央石英管组成，周围环绕着6盏高输出UV-C灯，置于一个较大的圆筒内，使空气在离开设备前能够再次循环经过UV-C灯。测试使用的是雾化的A/PR/8/34（H1N1）流感病毒。RNA提取和定量逆转录聚合酶链反应显示病毒通过了该原型设备。打开不同数量的灯可控制UV-C剂量。在低、中、高（100升/分钟）流量的污染气体条件下进行了生存力实验，分别激活6盏、4盏、2盏和1盏灯（测试了单次通过和再循环两种情况）。我们的数据显示，在高流量和低流量情况下，单盏灯（单次通过和再循环条件下）可使噬斑形成单位减少5个对数级。在100升/分钟时，单次通过的UV-C剂量计算为11.6毫焦/平方厘米，再循环时为104毫焦/平方厘米。该原型设备在高流量污染空气中杀灭雾化流感病毒方面显示出高效性。
