Khelifa R, Menezes J
J Virol. 1982 Feb;41(2):649-56. doi: 10.1128/JVI.41.2.649-656.1982.
As a direct approach to visualize Epstein-Barr virus (EBV) binding to its cellular receptors and to learn more on the nature of this binding, virus preparations were conjugated to fluorescein isothiocyanate and used to detect EBV receptors on lymphoid cells. Different enzymatic and chemical treatments were also applied either to the virus or to target cells or to both, and the effect of these treatments on virus binding was then examined. The results obtained show that: (i) EBV can be fluoresceinated without affecting its infectivity or cell binding ability, and the fluoresceinated virus represents an important tool to investigate the biology and nature of EBV interactions with its cellular receptors; (ii) the two virus strains (P3HR-1 and B95-8) share common receptors on Raji cells; (iii) protease treatment of EBV or target cells abrogates virus binding; (iv) EBV receptors regenerate after removal of the protease, and this regeneration is inhibited by cycloheximide or sucrose; (v) EBV particles bear concanavalin A receptors, and this lectin hinders the interaction of the virus with its cellular receptors; (vi) neuraminidase treatment, various monosaccharides, ovalbumin, and glycopeptides derived from EBV or cell surface do not inhibit virus binding. Taken together, the above data also demonstrate that some cellular and viral surface (glyco-) proteins are required for EBV binding to its targets.
作为一种直接观察爱泼斯坦-巴尔病毒（EBV）与其细胞受体结合并进一步了解这种结合本质的方法，将病毒制剂与异硫氰酸荧光素偶联，用于检测淋巴细胞上的EBV受体。还对病毒、靶细胞或两者都进行了不同的酶处理和化学处理，然后检查这些处理对病毒结合的影响。获得的结果表明：（i）EBV可以进行荧光素化而不影响其感染性或细胞结合能力，荧光素化病毒是研究EBV与其细胞受体相互作用的生物学和本质的重要工具；（ii）两种病毒株（P3HR-1和B95-8）在拉吉细胞上共享共同受体；（iii）对EBV或靶细胞进行蛋白酶处理会消除病毒结合；（iv）去除蛋白酶后EBV受体可再生，这种再生受到环己酰亚胺或蔗糖的抑制；（v）EBV颗粒带有刀豆球蛋白A受体，这种凝集素会阻碍病毒与其细胞受体的相互作用；（vi）神经氨酸酶处理、各种单糖、卵清蛋白以及源自EBV或细胞表面的糖肽均不抑制病毒结合。综上所述，上述数据还表明EBV与其靶标结合需要一些细胞和病毒表面（糖）蛋白。
