Li Peng, Huang Minli, Ma Yifan, Zhang Yongbin, Shi Changhong
Animal Laboratory Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University, Xi'an, 710032, Shaanxi, China.
Cancer Cell Int. 2024 Dec 31;24(1):438. doi: 10.1186/s12935-024-03628-3.
Tumor organoids have emerged as powerful tools for in vitro cancer research due to their ability to retain the structural and genetic characteristics of tumors. Nevertheless, the absence of a complete tumor microenvironment (TME) limits the broader application of organoid models in immunological studies. Given the critical role of immune cells in tumor initiation and progression, the co-culture model of organoids and peripheral blood mononuclear cells (PBMCs) may provide an effective platform for simulating the interactions between immune and tumor cells in vitro. This model stands as a robust instrument for dissecting the TME, elucidating the molecular interactions, and exploring the therapeutic applications of chimeric antigen receptor (CAR)-engineered lymphocytes, as well as other cancer treatment modalities. This review systematically evaluates the advantages and disadvantages of the co-culture model, identifies its technical bottlenecks, and proposes corresponding optimization strategies. By summarizing the latest research advancements in this co-culture model, our goal is to provide valuable insights for further model optimization and clinical application, thereby promoting immunological research and bridging the gap between experimental outcomes and clinical practice.
肿瘤类器官因其能够保留肿瘤的结构和遗传特征，已成为体外癌症研究的有力工具。然而，完整肿瘤微环境（TME）的缺失限制了类器官模型在免疫学研究中的更广泛应用。鉴于免疫细胞在肿瘤发生和发展中的关键作用，类器官与外周血单个核细胞（PBMC）的共培养模型可能为体外模拟免疫细胞与肿瘤细胞之间的相互作用提供一个有效平台。该模型是剖析TME、阐明分子相互作用以及探索嵌合抗原受体（CAR）工程化淋巴细胞及其他癌症治疗方式的治疗应用的有力工具。本综述系统评估了共培养模型的优缺点，确定了其技术瓶颈，并提出了相应的优化策略。通过总结该共培养模型的最新研究进展，我们的目标是为进一步的模型优化和临床应用提供有价值的见解，从而促进免疫学研究，并弥合实验结果与临床实践之间的差距。
