Park Ju-Hee, Hwang Yu-Na, Na Han-Heom, Kim Do-Yeon, Lee Hyo-Jun, Kwon Tae-Hyung, Park Jin-Sung, Kim Keun-Cheol
Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Republic of Korea.
Kangwon Center for System Imaging, Chuncheon, Republic of Korea.
J Pharmacopuncture. 2024 Dec 31;27(4):332-339. doi: 10.3831/KPI.2024.27.4.332.
To determine growth inhibitory and anti-cancer effects of Cannabigerol (CBG) in human colorectal cancer cells.
Anti-proliferative effect of CBG was examined using MTT assay and two colorectal cancer cells (SW480 and LoVo cells). Cell death ratio was analyzed using Annexin V/PI staining experiment. Cell cycle distribution was analyzed using flow cytometry. We also performed western blot analysis on apoptotic marker proteins.
CBG showed growth inhibitory effect in colorectal cancer cells using MTT assay. IC concentration of CBG was 34.89 μM in SW480 cells and 23.51 μM in LoVo cells. Annexin V/PI staining showed that CBG treatment increased apoptotic cells from 4.8% to 31.7% in SW480 cells and from 7.7% to 33.9% in LoVo cells. Flow cytometry confirmed that CBG increased sub G population via G arrest in both SW480 and LoVo cells. Western blot analysis showed that CBG increased expression levels of cell death-related proteins such as cleaved PARP-1, cleaved caspase 9, p53, and caspase 3.
CBG treatment shows antiproliferative activity and causes apoptosis of colorectal cancer cells, suggesting that CBG is applicable as a promising anticancer drug.
确定大麻二酚（CBG）对人结肠癌细胞的生长抑制和抗癌作用。
使用MTT法以及两种结肠癌细胞（SW480和LoVo细胞）检测CBG的抗增殖作用。采用膜联蛋白V/碘化丙啶（Annexin V/PI）染色实验分析细胞死亡率。使用流式细胞术分析细胞周期分布。我们还对凋亡标记蛋白进行了蛋白质印迹分析。
MTT法显示CBG对结肠癌细胞具有生长抑制作用。CBG在SW480细胞中的半数抑制浓度（IC）为34.89μM，在LoVo细胞中为23.51μM。Annexin V/PI染色显示，CBG处理使SW480细胞中的凋亡细胞从4.8%增加到31.7%，使LoVo细胞中的凋亡细胞从7.7%增加到33.9%。流式细胞术证实，CBG在SW480和LoVo细胞中均通过G期阻滞增加了亚G期细胞群体。蛋白质印迹分析表明，CBG增加了细胞死亡相关蛋白的表达水平，如裂解的PARP-1、裂解的半胱天冬酶9、p53和半胱天冬酶3。
CBG处理具有抗增殖活性并导致结肠癌细胞凋亡，表明CBG有望作为一种抗癌药物。
