Corder C N, Castillo R E, Nieder G L
J Pharmacol Methods. 1985 Feb;13(1):37-42. doi: 10.1016/0160-5402(85)90065-8.
The levels of norepinephrine, epinephrine, dopamine, adenosine triphosphate, and P-creatine were measured in whole mouse brain collected under two conditions: 1) the decapitated head was immediately plunged into liquid nitrogen (nonanoxic tissue); or 2) the tissue was allowed to remain anoxic before the quick-freezing procedure. The substrate levels were significantly decreased in brain anoxic for only 30 sec. The catecholamine levels in nonanoxic mouse brain appear to be higher than levels previously reported in brains collected with microwave irradiation or by decapitation with rapid dissection of tissue before it was frozen.
在两种条件下收集的全小鼠脑中测量去甲肾上腺素、肾上腺素、多巴胺、三磷酸腺苷和磷酸肌酸的水平：1）断头后立即将头部浸入液氮中（非缺氧组织）；或2）在快速冷冻程序之前，使组织保持缺氧状态。仅缺氧30秒的脑内底物水平就显著降低。非缺氧小鼠脑中的儿茶酚胺水平似乎高于先前报道的用微波辐射收集的脑或断头后在组织冷冻前快速解剖收集的脑内儿茶酚胺水平。
