Wang Na, Li Huimin, Wu Hao, Xia Zilin, Ren Dabing, Zhang Yunmei, Zhao Yan, Zhang Hong, Zhuang Ke, Yi Lunzhao
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.
Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, 650500, China; Department of Cardiology, First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650000, China.
J Pharm Biomed Anal. 2025 Mar 15;255:116651. doi: 10.1016/j.jpba.2024.116651. Epub 2024 Dec 26.
Myocardial infarction (MI) is a major cause of death worldwide. Exercise rehabilitation (ER) is a powerful tool to improve life quality and prognosis of MI patients. Herein, we developed an untargeted metabolomics combined with lipidomics method to qualitatively and quantitatively detect metabolites in plasma. A total of 475 metabolites were annotated according to MS, MS/MS, and quantified by internal standard method. Moreover, medical statistical methods combined with chemometrics were used for metabolomics data mining and interpretation of clinical issues (matched Cohort 1, n = 90, Cohort 2, n = 6). The results illustrated that abnormal lipid metabolism is the most significant metabolic disorder for MI patients. And, three metabolic pathways, bile secretion, HIF-1 signaling pathway, and glutathione metabolism were uncovered in MI patients. Furthermore, glutamine, Phenylacetylglutamine (PAGln) and lysophosphatidylcholine (LPCs) were revealed as the essential biomarkers for ER of MI patients. Our findings revealed the metabolic landscape of MI and metabolic alterations after ER, will underlay potential applications of plasma metabolites in the detection of MI and optimization of ER program.
心肌梗死（MI）是全球主要的死亡原因。运动康复（ER）是改善MI患者生活质量和预后的有力工具。在此，我们开发了一种非靶向代谢组学结合脂质组学方法，用于定性和定量检测血浆中的代谢物。根据质谱（MS）、串联质谱（MS/MS）对总共475种代谢物进行了注释，并采用内标法进行定量。此外，将医学统计方法与化学计量学相结合，用于代谢组学数据挖掘和临床问题解读（匹配队列1，n = 90；队列2，n = 6）。结果表明，脂质代谢异常是MI患者最显著的代谢紊乱。并且，在MI患者中发现了三条代谢途径，即胆汁分泌、缺氧诱导因子-1（HIF-1）信号通路和谷胱甘肽代谢。此外，谷氨酰胺、苯乙酰谷氨酰胺（PAGln）和溶血磷脂酰胆碱（LPCs）被揭示为MI患者运动康复的关键生物标志物。我们的研究结果揭示了MI的代谢概况以及运动康复后的代谢变化，将为血浆代谢物在MI检测和运动康复方案优化中的潜在应用奠定基础。
