Goulart Taynara Santos, Prado Maick Meneguzzo, Tieppo Giulia Celine, Fischer Bruna Venzke, Schuldt Daniela Peressoni Vieira, Coelho Beatriz Serrato, Bortoluzzi Eduardo Antunes, da Silveira Teixeira Cleonice, da Fonseca Roberti Garcia Lucas, de Almeida Josiane
Department of Dentistry - Endodontics Division, Health Sciences Center, Federal University of Santa Catarina (UFSC), Campus Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, 88040-900, Brazil.
Department of Chemical Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil.
Odontology. 2025 Jan 2. doi: 10.1007/s10266-024-01047-4.
This in vitro research assessed the influence of the instrument kinematics (rotary and reciprocating) and the apical preparation limit on the root canal disinfection and apical bacterial extrusion. After 21 days of Enterococcus faecalis biofilm formation in 72 mesial root canals of mandibular molars, the root canals were distributed into 2 groups (n = 36) according to the systems used for preparation: ProDesign S and Reciproc. The groups were redistributed according to the limit of apical preparation (n = 11): (a) 1 mm up to the apical foramen (TL-1); (b) at the apical foramen (TL = 0); (c) 1 mm beyond the apical foramen (TL + 1). After preparation, the remaining biofilm adhered to the dentin walls at the apical third was removed by sonication. The aliquots of bacterial suspension released, and the irrigating solution leaked through the apical foramen during preparation were plated for colony-forming units (CFUs) counting. Data were statistically assessed by the Kruskal-Wallis and Dunn tests (α = 5%). Both systems promoted decontamination of the apical third, regardless the limit of apical preparation (p > 0.05). A larger quantity of bacteria was extruded from the root canals prepared 1 mm beyond the apical foramen, regardless the instrumentation kinematics (p < 0.05). The apical third of the root canal was efficiently decontaminated after preparation, regardless the instrumentation kinematics and the apical limit. However, a larger quantity of bacterial extrusion was observed when preparation was performed beyond the anatomical root canal limits.
这项体外研究评估了器械运动方式（旋转和往复）以及根尖预备极限对根管消毒和根尖细菌挤出的影响。在72颗下颌磨牙的近中根管中形成粪肠球菌生物膜21天后，根据预备所用系统将根管分为2组（n = 36）：ProDesign S组和Reciproc组。再根据根尖预备极限将这些组重新分配（n = 11）：（a）距根尖孔1 mm（TL-1）；（b）在根尖孔处（TL = 0）；（c）超出根尖孔1 mm（TL + 1）。预备后，通过超声处理去除根尖三分之一处牙本质壁上残留的生物膜。对预备过程中释放的细菌悬液等分试样以及通过根尖孔渗漏的冲洗液进行菌落形成单位（CFU）计数接种。数据采用Kruskal-Wallis检验和Dunn检验进行统计学评估（α = 5%）。无论根尖预备极限如何，两种系统均能促进根尖三分之一处的去污（p > 0.05）。无论器械运动方式如何，从超出根尖孔1 mm预备的根管中挤出的细菌数量更多（p < 0.05）。无论器械运动方式和根尖极限如何，根管预备后根尖三分之一处均能有效去污。然而，当预备超出解剖学根管极限时，观察到更多的细菌被挤出。
