Kamilari E, O'Connor P M, Farias F Miceli de, Johnson C N, Buttimer C, Deliephan A, Hill D, Fursenko O, Wiese J, Stanton C, Hill C, Ross R P
School of Microbiology, University College Cork, Cork, County Cork, Ireland.
APC Microbiome Ireland, Cork, Ireland.
Appl Environ Microbiol. 2025 Jan 31;91(1):e0194224. doi: 10.1128/aem.01942-24. Epub 2024 Dec 31.
APC 4099, isolated from bees' gut, has been identified as a promising candidate for food biopreservation. Antimicrobial activity screening revealed a broad-spectrum inhibition potential, ranging from gram-positive pathogenic bacteria to fungi responsible for food spoilage. Genomic analysis identified biosynthetic gene clusters coding for several antimicrobial peptides and secondary metabolites. Specifically, a novel, anionic, 6 kDa circular bacteriocin, named safencin E, was detected, showing 52.5% similarity to butyrivibriocin AR10. Additionally, gene clusters coding for the biosynthesis of bacteriocins such as pumilarin and plantazolicin and biosynthetic pathways for secondary metabolites, including pumilacidin A, bacilysin, and bacillibactin, were identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis detected molecular masses correlating to safencin E, plantazolicin, pumilarin, and pumilacidin A from the cell-free supernatant, cell extracts, or both. Overall, the broad-spectrum antimicrobial activity of APC 4099 indicates that this strain is a promising candidate for the biological control of food ecosystems and thus has the potential to enhance food safety.
The present article highlights the importance of the strain APC 4099 as a potential biocontrol agent. The strain possesses biosynthetic gene clusters coding for various antimicrobial peptides and secondary metabolites, including a novel circular bacteriocin, safencin E, and the bacteriocins pumilarin and plantazolicin. This diversity in the production of antimicrobial peptides renders the producer with broad-spectrum antimicrobial activity, ranging from gram-positive pathogenic and spoilage bacteria to spoilage molds. Considering that 1.3 billion tons of food appropriate for human consumption is lost or wasted annually, identifying strains or novel antimicrobial peptides capable of biopreservation is highly relevant. This strain and its bioactive compounds offer a solution to this global problem as biocontrol agents for food ecosystems against spoilage and pathogenic microbes.
从蜜蜂肠道中分离出的APC 4099已被确定为食品生物保鲜的一个有前景的候选菌株。抗菌活性筛选显示其具有广谱抑制潜力，范围从革兰氏阳性病原菌到导致食物变质的真菌。基因组分析确定了编码几种抗菌肽和次生代谢物的生物合成基因簇。具体而言，检测到一种名为safencin E的新型阴离子6 kDa环状细菌素，与丁酸弧菌菌素AR10有52.5%的相似性。此外，还鉴定了编码诸如pumilarin和plantazolicin等细菌素生物合成的基因簇以及包括pumilacidin A、杆菌溶素和杆菌铁载体素等次生代谢物的生物合成途径。基质辅助激光解吸电离飞行时间质谱分析从无细胞上清液、细胞提取物或两者中检测到与safencin E、plantazolicin、pumilarin和pumilacidin A相关的分子量。总体而言，APC 4099的广谱抗菌活性表明该菌株是食品生态系统生物防治的一个有前景的候选菌株，因此有潜力提高食品安全。
本文强调了菌株APC 4099作为潜在生物防治剂的重要性。该菌株拥有编码各种抗菌肽和次生代谢物的生物合成基因簇，包括一种新型环状细菌素safencin E以及细菌素pumilarin和plantazolicin。抗菌肽产生的这种多样性使生产者具有广谱抗菌活性，范围从革兰氏阳性病原菌和腐败细菌到腐败霉菌。鉴于每年有13亿吨适合人类消费的食物损失或浪费，鉴定能够进行生物保鲜的菌株或新型抗菌肽具有高度相关性。该菌株及其生物活性化合物作为食品生态系统对抗腐败和致病微生物的生物防治剂，为这一全球性问题提供了一个解决方案。
