Shodmonova Mukhlisa K, Muhammadova Dono A, Aytenov Ilkham S, Isokulov Marufbek Z, Bozorov Tohir A, Zhang Daoyuan, Abduraimov Ozodbek S, Murodova Sojida M, Melikuziev Fazliddin A, Ochilov Bekhruz O, Meliev Sodir K
Laboratory of Molecular and Biochemical Genetics, Institute of Genetics and Plants Experimental Biology, Uzbek Academy of Sciences, Tashkent Region, Yukori-Yuz, Kibray 111226, Uzbekistan.
Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Microorganisms. 2025 May 20;13(5):1161. doi: 10.3390/microorganisms13051161.
Plant-associated bacteria play a crucial role in protecting plants from pathogens, yet the diversity and antagonistic potential of these bacteria across different plant species remain underexplored, especially in central Asia. To investigate the competitive dynamics between phytopathogenic fungi and plant-associated bacteria, we collected stem and root samples from 50 plant species across nine regions of Uzbekistan. A total of 3355 bacterial isolates were obtained (1896 from roots and 1459 from shoots) and screened for antifungal activity against six fungal pathogens, resulting in 432 antagonistic isolates. These were identified through 16S rDNA sequencing, revealing 65 bacterial species across three phyla: Firmicutes, Proteobacteria, and Actinobacteria, predominantly in the respective families , , and The plant hosted the highest diversity of antagonists (26 species), while other species harbored fewer. Plant species showed strong associations with specific bacterial communities, with 14 plant species each hosting unique antagonists. Enzymatic profiling revealed functional diversity, with species producing protease, cellulase, and lipase activities, while species excelled in xylanase, glucanase, and cellobiase production. 9r-29 stood out by producing all six enzymes. These findings underscore the ecological diversity and biocontrol potential of plant-associated bacteria in natural ecosystems, offering promising candidates for sustainable plant protection strategies.
与植物相关的细菌在保护植物免受病原体侵害方面发挥着关键作用，然而，这些细菌在不同植物物种间的多样性和拮抗潜力仍未得到充分探索，尤其是在中亚地区。为了研究植物病原真菌与植物相关细菌之间的竞争动态，我们从乌兹别克斯坦九个地区的50种植物中采集了茎和根样本。共获得3355株细菌分离株（1896株来自根，1459株来自茎），并对其针对六种真菌病原体的抗真菌活性进行了筛选，得到432株拮抗分离株。通过16S rDNA测序对这些分离株进行鉴定，结果显示它们分属于三个门的65种细菌：厚壁菌门、变形菌门和放线菌门，主要分布在各自的科 、 和 中。 植物拥有最高的拮抗物多样性（26种），而其他物种含有的拮抗物较少。植物物种与特定细菌群落表现出强烈的关联性，有14种植物各自拥有独特的拮抗物。酶谱分析揭示了功能多样性， 种细菌产生蛋白酶、纤维素酶和脂肪酶活性，而 种细菌在木聚糖酶、葡聚糖酶和纤维二糖酶的产生方面表现出色。9r - 29通过产生所有六种酶而脱颖而出。这些发现强调了自然生态系统中与植物相关细菌的生态多样性和生物防治潜力，为可持续植物保护策略提供了有前景的候选方案。
