Han Shipeng, Wang Xiaoqi, Han Hui, Wang Da, He Yunzhuan
College of Plant Protection, Shandong Agricultural University, Taian, Shandong 271000, P. R. China.
College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071000, P. R. China.
J Agric Food Chem. 2025 Jan 15;73(2):1122-1130. doi: 10.1021/acs.jafc.4c08653. Epub 2025 Jan 2.
is a major migratory invasive pest and is of global concern. Vitellogenesis, a crucial process for population multiplication in oviparous insects, is regulated by endocrine hormones. In this study, three primary responders to JH signaling, the JH receptor gene , and the downstream transcription factor and , were first cloned and identified. RNA interference results showed that silencing significantly down-regulated the transcription levels of and , as well as the key reproductive genes () and (). Similarly, silencing and also inhibited the transcription of and . Silencing of , , and genes resulted in blocked ovarian development and a significant decrease in reproduction. These findings confirm that and comediate the action of the JH- signaling pathway in vitellogenesis of , providing new targets and insights for pest control.
是一种主要的迁飞性入侵害虫，受到全球关注。卵黄发生是卵生昆虫种群繁殖的关键过程，受内分泌激素调节。在本研究中，首次克隆并鉴定了对保幼激素（JH）信号的三个主要应答者，即JH受体基因、下游转录因子和。RNA干扰结果表明，沉默显著下调了和的转录水平，以及关键生殖基因（）和（）的转录水平。同样，沉默和也抑制了和的转录。、和基因的沉默导致卵巢发育受阻和繁殖力显著下降。这些发现证实，和共同介导了JH信号通路在卵黄发生中的作用，为害虫防治提供了新的靶点和见解。
