Li I C, Wu S C, Fu J, Chu E H
Mutat Res. 1985 Mar;149(1):127-32. doi: 10.1016/0027-5107(85)90017-x.
Unequal growth rates between mutant and wild-type cells in a large population constitute a problem for the estimation of mutation rate. Over a period of cell growth, a selective advantage of one cell type over the other might lead to considerable error in the estimation of mutation rate if equal growth rates are assumed. In this study, we propose a formula and apply it to the estimation of spontaneous mutation rate in a growing population of Chinese hamster V79 cells in which ouabain-resistant mutant cells exhibit a slower growth rate than the wild-type cells. The formula is a generalization of that previously presented by Armitage (1953), and this is the first attempt to apply the deterministic approach for mutation rate estimation to cultured mammalian cells. The value of the estimated rate is compared with that derived from a parallel experiment using the fluctuation test of Luria and Delbrück (1943). The limitations and advantages of taking the deterministic approach to mutation rate estimation in mammalian cell systems are discussed.
在一个大群体中，突变细胞和野生型细胞之间生长速率的不平等给突变率的估计带来了问题。在细胞生长过程中，如果假设两种细胞类型的生长速率相等，那么一种细胞类型相对于另一种细胞类型的选择性优势可能会导致突变率估计出现相当大的误差。在本研究中，我们提出了一个公式，并将其应用于中国仓鼠V79细胞生长群体中自发突变率的估计，其中哇巴因抗性突变细胞的生长速率比野生型细胞慢。该公式是对Armitage（1953年）之前提出的公式的推广，这是首次尝试将确定性方法用于突变率估计，并应用于培养的哺乳动物细胞。将估计速率的值与使用Luria和Delbrück（1943年）的波动试验的平行实验得出的值进行比较。讨论了在哺乳动物细胞系统中采用确定性方法估计突变率的局限性和优点。
