Witt Emily, Petersen Emily B, Alzayadneh Eyas, Courtney Ryan J, Brouillette Marc J, Wang Qi, Sakyi Maxwell Y, Watson Nicole A D, Rivas Dominic, Bi Jianling, Culver Lindsey, Balk Kyle, Reis Colin, Uaroon Slyn, McClintic Kaitlyn A, Hatfield Samual, Worthington Kristan S, Sander Edward A, Traverso Giovanni, Otterbein Leo E, Goetz Jessica E, Fredericks Douglas C, Byrne James D
Department of Biomedical Engineering, University of Iowa, Iowa City, Iowa 52242, United States.
Department of Radiation Oncology, University of Iowa, Iowa City, Iowa 52242, United States.
Biomacromolecules. 2025 Jan 13;26(1):201-208. doi: 10.1021/acs.biomac.4c00904. Epub 2025 Jan 2.
Tissue repair is often impaired in pathological states, highlighting the need for innovative wound-healing technologies. This study introduces composite hyaluronic acid gas-entrapping materials (GEMs) delivering carbon monoxide (CO) to promote wound healing in pigs. These composite materials facilitate burst release followed by sustained release of CO over 48 h. In a porcine full-thickness wound model, CO-GEMs significantly accelerated wound closure compared to the standard-of-care dressing (Tegaderm). Wound area closure with CO-GEMs was 68.6% vs 56.8% on day 14, 41.0% vs 25.1% on day 28, and 26.9% vs 11.8% on day 42, effectively reducing healing time by 14 days. Histological analysis revealed increased epithelialization and neovascularization with reduced inflammation. These findings demonstrate the potential of CO-GEMs as a topical therapeutic to enhance tissue repair in clinically relevant models, supporting further testing for wound-healing applications.
在病理状态下，组织修复常常受损，这凸显了创新伤口愈合技术的必要性。本研究介绍了一种复合透明质酸气体包裹材料（GEMs），其可释放一氧化碳（CO）以促进猪的伤口愈合。这些复合材料能够实现突发释放，随后在48小时内持续释放CO。在猪的全层伤口模型中，与标准护理敷料（Tegaderm）相比，CO-GEMs显著加速了伤口闭合。在第14天，使用CO-GEMs的伤口面积闭合率为68.6%，而使用标准护理敷料的为56.8%；在第28天，分别为41.0%和25.1%；在第42天，分别为26.9%和11.8%，有效缩短愈合时间14天。组织学分析显示，上皮化和新血管形成增加，炎症减轻。这些发现表明，在临床相关模型中，CO-GEMs作为一种局部治疗方法具有增强组织修复的潜力，支持进一步开展伤口愈合应用测试。
