Feng Yu, Zhang Xingyu, Zhang Caiwei, Xu Haozhe, Ji Xiaoming, Wang Jianfang, Wu Peng, Qian Feiyue, Chen Chongjun, Shen Yaoliang, Liu Wenru
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2025 Mar;419:132033. doi: 10.1016/j.biortech.2024.132033. Epub 2024 Dec 31.
In this study, waste iron scraps (WIS) were exerted to alleviate sulfide inhibition on anammox bacteria and promote anammox nitrogen removal from sulfide-containing wastewater.Short-term batch experiments showed that WIS-addition led to the anammox bacteria activity increasing by 124.8 % at an initial sulfide concentration of 40 mgS/L. During the long-term experiments, the nitrogen removal rate (NRR) reached to 8.76 kg/(m·d) in the WIS-added reactor, while the maximum NRR was only 3.77 ± 0.31 kg/(m·d) in the non-WIS reactor. In contrast to anammox bacteria development in the non-WIS reactor, the relative abundance of Candidatus Kuenenia (1.4-3.7 %) declined significantly in the WIS-added reactor, but novel potential anammox bacteria Brocadiaceae_unclassified (60.1 %-78.6 %) were highly enriched. Overall, the experimental evidence suggested that WIS-addition not only mitigated the sulfide inhibition on anammox bacteria, but also promoted novel anammox bacteria proliferation. The findings of this work provide a promising solution for wide engineering applications of anammox treating sulfide-containing wastewater.
在本研究中，采用废铁屑（WIS）来缓解硫化物对厌氧氨氧化菌的抑制作用，并促进从含硫化物废水中去除厌氧氨氧化氮。短期批次实验表明，在初始硫化物浓度为40 mgS/L时，添加WIS使厌氧氨氧化菌活性提高了124.8%。在长期实验中，添加WIS的反应器中氮去除率（NRR）达到8.76 kg/(m³·d)，而未添加WIS的反应器中最大NRR仅为3.77±0.31 kg/(m³·d)。与未添加WIS的反应器中厌氧氨氧化菌的生长情况相比，添加WIS的反应器中“库氏假单胞菌”（Candidatus Kuenenia）的相对丰度（1.4%-3.7%）显著下降，但新型潜在厌氧氨氧化菌“未分类的布罗卡德氏菌科”（Brocadiaceae_unclassified，60.1%-78.6%）高度富集。总体而言，实验证据表明，添加WIS不仅减轻了硫化物对厌氧氨氧化菌的抑制作用，还促进了新型厌氧氨氧化菌的增殖。本研究结果为厌氧氨氧化处理含硫化物废水的广泛工程应用提供了一个有前景的解决方案。
