用于双通道体内成像的稳定且生物相容的短波红外纳米带。
A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging.
作者信息
Yao Cheng, Wei Ruwei, Luo Xiao, Zhou Jie, Zhang Xiaodong, Lu Xicun, Dong Yan, Chu Ruofan, Sun Yuxin, Wang Yu, Xia Wencheng, Qu Dahui, Liu Cong, Ren Jun, Ge Guangbo, Chen Jinquan, Qian Xuhong, Yang Youjun
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Shanghai Key Laboratory of Chemical Biology, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
出版信息
Nat Commun. 2025 Jan 2;16(1):4. doi: 10.1038/s41467-024-55445-x.
The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains challenging. We propose a crystal-aided aggregate synthesis (CAASH) approach to introduce a layer of rationality for the development of J-aggregate and the successful development of a water-soluble SWIR JV-aggregate with a bisbenzannulated silicon rhodamine scaffold (ESi5). The resulting SWIR-aggregates exhibit excellent stabilities toward organic solvents, pH, sonication, photobleaching, thiols, and endogenous oxidative species. Notably, the aggregates have a high structure-dependent melting temperature of ca. 330-335 K. In fact, the heating/annealing process can be exploited to reduce aggregation disorder. The aggregates are biocompatible and have broad potential in in vivo fluorescence and photoacoustic imaging and more.
短波红外（SWIR）区域是下一代生物成像的理想光谱窗口，可提高穿透深度并降低光毒性。通过超分子染料聚集也可以实现SWIR光谱活性。不幸的是，染料聚集的发展仍然具有挑战性。我们提出了一种晶体辅助聚集合成（CAASH）方法，为J-聚集体的开发引入一层合理性，并成功开发了一种具有双苯并硅罗丹明支架（ESi5）的水溶性SWIR J-聚集体。所得的SWIR聚集体对有机溶剂、pH值、超声处理、光漂白、硫醇和内源性氧化物质表现出优异的稳定性。值得注意的是，这些聚集体具有约330-335 K的高度依赖结构的熔化温度。事实上，加热/退火过程可用于减少聚集无序。这些聚集体具有生物相容性，在体内荧光和光声成像等方面具有广泛的潜力。
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