State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Dongchuan Road 500, Shanghai 200241, China.
J Am Chem Soc. 2023 Jun 7;145(22):12013-12022. doi: 10.1021/jacs.3c00594. Epub 2023 May 22.
The shortwave infrared (SWIR) spectral region beyond 1200 nm offers optimal tissue penetration depth and has broad potential in diagnosis, therapy, and surgery. Here, we devised a novel class of fluorochromic scaffold, i.e., a tetra-benzannulated xanthenoid (). absorbs/emits maximally at 1204/1290 nm in CHCl and exhibits an unparalleled molar absorptivity of 3.91 × 10 cm M and high transparency to light at 400-900 nm. It also exhibited high resistance toward both photobleaching and symmetry breaking due to its unique structural rigidity. It is feasible for in vivo bioimaging and particularly suitable to couple with the shorter-wavelength analogues for high-contrast multiplexing. High-contrast dual-channel intraoperative imaging of the hepatobiliary system and three-channel in vivo imaging of the intestine, the stomach, and the vasculature were showcased. is a benchmark fluorochrome for facile biomedical exploitation of the SWIR region beyond 1200 nm.
短波红外（SWIR）光谱区域在 1200nm 以上，具有最佳的组织穿透深度，在诊断、治疗和手术方面具有广泛的应用潜力。在这里，我们设计了一类新型的荧光染料支架，即四苯并蒽酮（）。在 CHCl 中最大吸收/发射波长为 1204/1290nm，摩尔消光系数高达 3.91×10cmM，在 400-900nm 范围内对光具有高透明度。由于其独特的结构刚性，它还表现出对光漂白和对称性破坏的高抗性。它适用于体内生物成像，特别是与较短波长的类似物结合，用于高对比度的多重标记。我们展示了用于肝胆系统的高对比度双通道术中成像，以及用于肠道、胃和脉管系统的三通道体内成像。是一种基准荧光染料，可方便地在 1200nm 以上的 SWIR 区域进行生物医学应用。
