Safari-Gezaz Meysam, Parhizkar Mojtaba, Asghari Elnaz
Department of Condensed Matter Physics, Faculty of Physics, University of Tabriz, 29 Bahman Blvd., Tabriz, Iran.
Department of Physical Chemistry, Faculty of Chemistry, University of Tabriz, 29 Bahman Blvd., Tabriz, Iran.
Sci Rep. 2025 Jan 2;15(1):149. doi: 10.1038/s41598-024-84055-2.
Hydroxyapatite (HA) is an engineered biomaterial that closely resembles the hard tissue composition of humans. Biological HA is commonly non-stoichiometric and features lower crystallinity and higher solubility than stoichiometric HA. The chemical compositions of these biomaterials include calcium (Ca), phosphorus (P), and trace amounts of various ions such as magnesium (Mg), zinc (Zn), and strontium (Sr). Significantly, these ions are essential for the metabolic processes of hard tissues. This study involved the application of Co-doped HA coatings at different concentrations (5%, 10%, and 20% by weight) onto Ti-6Al-4V, utilizing the spin-coating method. The FTIR, XRD, FESEM, EDS, and AFM techniques were utilized to analyze the coated substrates. Tetraethyl orthosilicate (TEOS (T)) was employed as a binding agent to enhance adhesion and reduce surface cracks in the coating. The adhesion strength of coatings applied to Ti-6Al-4V was assessed for use in biomedical applications. Polarization and electrochemical impedance spectroscopy (EIS) studies in a simulated body fluid (SBF) solution were conducted to evaluate the corrosion behavior of the coatings. The corrosion behavior of the coated samples increased significantly compared to the substrate. The 10Co/HA/T coating demonstrated the highest charge transfer resistance (R) value of 13.40 MΩ × cm, whereas the uncoated substrate exhibited the lowest R of 0.14 MΩ × cm. A cell viability assay was conducted utilizing MG-63 cells for the Ti-6Al-4V and coatings, which prepared coatings demonstrated outstanding biocompatibility. Based on this study, the 10Co/HA/T coating was identified as the most promising sample. These findings suggest that surface modification of Ti-6Al-4V through Co-doped HA coatings offers a viable strategy for enhancing its performance in biomedical applications.
羟基磷灰石（HA）是一种人工合成生物材料，与人体硬组织成分极为相似。生物HA通常是非化学计量的，与化学计量的HA相比，其结晶度较低，溶解度较高。这些生物材料的化学成分包括钙（Ca）、磷（P）以及微量的各种离子，如镁（Mg）、锌（Zn）和锶（Sr）。重要的是，这些离子对硬组织的代谢过程至关重要。本研究采用旋涂法，将不同浓度（重量百分比分别为5%、10%和20%）的钴掺杂HA涂层涂覆在Ti-6Al-4V上。利用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、场发射扫描电子显微镜（FESEM）、能谱仪（EDS）和原子力显微镜（AFM）技术对涂覆后的基底进行分析。使用正硅酸乙酯（TEOS（T））作为粘结剂，以增强涂层的附着力并减少表面裂纹。评估了涂覆在Ti-6Al-4V上的涂层的附着力强度，以用于生物医学应用。在模拟体液（SBF）溶液中进行极化和电化学阻抗谱（EIS）研究，以评估涂层的腐蚀行为。与基底相比，涂覆样品的腐蚀行为显著增加。10Co/HA/T涂层的电荷转移电阻（R）值最高，为13.40MΩ·cm，而未涂覆的基底的R值最低，为0.14MΩ·cm。利用MG-63细胞对Ti-6Al-4V和涂层进行细胞活力测定，结果表明制备的涂层具有出色的生物相容性。基于本研究，10Co/HA/T涂层被确定为最有前景的样品。这些发现表明，通过钴掺杂HA涂层对Ti-6Al-4V进行表面改性，为提高其在生物医学应用中的性能提供了一种可行的策略。
