Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, 199 Ren'ai Road, Suzhou 215123, China.
Department of Orthopedic, Spinal Pain Research Institute, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110687. doi: 10.1016/j.msec.2020.110687. Epub 2020 Jan 23.
Electronic structure and bioactivity of calcium phosphate (CaP) coatings on Ti-based anodic nanotubes are investigated. Nanotubes on pure Ti and Ti-6Al-4V alloy, respectively, are used as substrates for CaP deposition. The CaP coatings are formed by first growing a seeding CaP layer using alternative immersion (AIM) treatment followed by crystallization in Dulbecco's phosphate-buffered saline (DPBS). CaP coatings formed on both Ti and Ti-6Al-4V substrates are found containing a variety of bioactive CaP species, such as hydroxyapatite (HA), amorphous CaP (ACP), octacalcium phosphate (OCP), and dicalcium phosphate dihydrate (DCPD). The compositions of the coatings during the nucleation and crystallization processes are tracked and analyzed using X-ray absorption near-edge structure (XANES). The variation of CaP species in the resulted coatings are found strongly dependent on the choice of metal substrates, which leads to different bioactivities. By comparing the proliferation and differentiation of osteoblast cells (MC3T3-E1) on the CaP coatings, correlations between CaP species and their bioactivities are established.
研究了钛基阳极氧化纳米管上磷酸钙（CaP）涂层的电子结构和生物活性。分别以纯钛和 Ti-6Al-4V 合金作为 CaP 沉积的基底来制备纳米管。采用交替浸涂（AIM）处理先在基底上生长一层种子 CaP 层，然后在 Dulbecco 的磷酸盐缓冲盐水（DPBS）中结晶。结果表明，在 Ti 和 Ti-6Al-4V 基底上形成的 CaP 涂层均含有多种生物活性 CaP 物质，如羟基磷灰石（HA）、无定形 CaP（ACP）、八钙磷酸盐（OCP）和二水合磷酸二钙（DCPD）。使用 X 射线吸收近边结构（XANES）跟踪和分析了成核和结晶过程中涂层的组成。发现所得涂层中 CaP 物质的变化强烈依赖于金属基底的选择，这导致了不同的生物活性。通过比较 CaP 涂层上成骨细胞（MC3T3-E1）的增殖和分化，建立了 CaP 物质与其生物活性之间的相关性。
