Bernabe Maria Clara Mendes, Fonseca Belchiolina Beatriz, Silva Maysa Vitória Cunha, Pedrosa Isabelle Ezequiel, Silva Michelle Borges, Sommerfeld Simone, de Sousa Amanda Luiza Pereira, de Leva Resende Bruna Candelori, Sousa Ana Carolina Prado, Dos Santos Freitas Andria, de Azevedo Vasco Ariston Carvalho, Guédon Éric, da Silva Elisa Sant'Anna Monteiro
School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil.
Graduate Program in Genetic and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Vet Res Commun. 2025 Jan 2;49(1):61. doi: 10.1007/s11259-024-10626-3.
Endometritis is one of the main reproductive disorders in mares and due to the increasing prevalence of antibiotic resistance, the use of probiotics in the prevention and treatment of endometritis in mares has gained interest, given their potential to restore and maintain a healthy uterine microbiota. Therefore, the aim of this study was to evaluate the antimicrobial properties of total metabolites of Lactobacillus acidophilus (LA) and Lactiplantibacillus plantarum (LP) against common equine endometrial pathogenic bacteria in vitro (Acinetobacter baumannii, Escherichia coli (1), Escherichia coli (2), Escherichia coli (3), Escherichia coli (4), Enterobacter cloacae, Streptococcus equi, Staphylococcus warneri, Actinobacillus equi and Klebesiella pneumoniae), as well as to assess their low molecular weight metabolites (loM) and extracellular vesicle (EVs) inhibition capacity over a multidrug-resistant E. coli isolated from mares with clinical endometritis. The total metabolites of LA showed better inhibition on A. baumannii, E. coli (1) and E. cloacae, while those of LP inhibited E. coli (4), S. equi and A. equi. Besides total metabolites, loM of LA and LP can inhibit E. coli. LA EVs were more effective in preventing E. coli (2) compared to LA loM, while LP EVs presented inhibition but below 90%. The use of LA and LP in the mare's uterus may be an interesting approach to controlling endometritis. In addition to metabolites, EVs can contribute to the inhibition of multidrug-resistant E. coli.
子宫内膜炎是母马主要的生殖系统疾病之一。鉴于抗生素耐药性日益普遍，由于益生菌具有恢复和维持子宫健康微生物群的潜力，其在母马子宫内膜炎预防和治疗中的应用受到了关注。因此，本研究的目的是评估嗜酸乳杆菌（LA）和植物乳杆菌（LP）的总代谢产物对常见马子宫内膜病原菌（鲍曼不动杆菌、大肠杆菌（1）、大肠杆菌（2）、大肠杆菌（3）、大肠杆菌（4）、阴沟肠杆菌、马链球菌、华纳葡萄球菌、马放线杆菌和肺炎克雷伯菌）的体外抗菌特性，以及评估它们的低分子量代谢产物（loM）和细胞外囊泡（EVs）对从患有临床子宫内膜炎的母马分离出的多重耐药大肠杆菌的抑制能力。LA的总代谢产物对鲍曼不动杆菌、大肠杆菌（1）和阴沟肠杆菌表现出更好的抑制作用，而LP的总代谢产物则抑制大肠杆菌（4）、马链球菌和马放线杆菌。除总代谢产物外，LA和LP的loM均可抑制大肠杆菌。与LA的loM相比，LA的EVs在预防大肠杆菌（2）方面更有效，而LP的EVs有抑制作用，但低于90%。在母马子宫中使用LA和LP可能是控制子宫内膜炎的一种有趣方法。除代谢产物外，EVs也有助于抑制多重耐药大肠杆菌。
