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相关细胞外囊泡的转录组分析揭示了小RNA货物的存在。

Transcriptome Profiling of Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo.

作者信息

Joshi Bishnu, Singh Bhupender, Nadeem Aftab, Askarian Fatemeh, Wai Sun Nyunt, Johannessen Mona, Hegstad Kristin

机构信息

Department of Medical Biology, Research Group for Host-Microbe Interactions, UiT The Arctic University of Norway, Tromsø, Norway.

Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.

出版信息

Front Mol Biosci. 2021 Jan 13;7:566207. doi: 10.3389/fmolb.2020.566207. eCollection 2020.

DOI:10.3389/fmolb.2020.566207
PMID:33521050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7838569/
Abstract

Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction.

摘要

细菌细胞外囊泡（EVs）在细菌致病过程中起着至关重要的作用。然而，迄今为止，由机会致病菌释放的EVs中的小RNA货物尚未得到表征。在这里，我们揭示了在添加亚抑菌剂量万古霉素以模拟感染条件的缺铁细菌培养基中培养的MSSA476分泌的小RNA与EVs之间的关联。对完整的经核糖核酸酶处理的EVs进行共聚焦显微镜分析表明，RNA与EV颗粒相关联。对EV相关RNA进行转录组学分析，随后进行生物信息学分析，发现了潜在的基因调控小RNA和高水平的转运RNA（tRNAs）。在与EV相关的富集小RNA中，有SsrA、RsaC和RNAIII。我们的发现为EV相关RNA作为宿主-病原体相互作用调节剂的潜在作用带来了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/ce88f028fa27/fmolb-07-566207-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/1d3de511e684/fmolb-07-566207-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/536f34771138/fmolb-07-566207-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/064b426c286e/fmolb-07-566207-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/ce88f028fa27/fmolb-07-566207-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/1d3de511e684/fmolb-07-566207-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/536f34771138/fmolb-07-566207-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/064b426c286e/fmolb-07-566207-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/7838569/ce88f028fa27/fmolb-07-566207-g0004.jpg

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