Ghosh Nabanita, Biswas Arup Ratan, Chakraborty Arindam, Ganguli Arnab
Department of Microbiology, Techno India University, West Bengal EM-4 Sector-V, Salt Lake City, Kolkata, West Bengal, 700091, India.
Department of Chemistry, Techno India University, West Bengal EM-4 Sector-V, Salt Lake City, Kolkata, West Bengal, 700091, India.
Folia Microbiol (Praha). 2025 Jan 2. doi: 10.1007/s12223-024-01238-0.
This study investigated the application of mixed biofilms formed by two Pseudomonas strains (NAA22 and NAA23) for bio-decolorization of malachite green (MG) dye. The isolated strains displayed biofilm formation and MG decolorization capabilities. Mixed biofilms exhibited significantly greater biofilm formation and MG decolorization (94.3%) compared to individual strains, suggesting synergistic interactions. This decolorization efficiency surpassed previously reported values for single strain decolorization. The mixed biofilms tolerated a broad range of temperatures (20-40 °C) and pH (5-9), with optimal decolorization at neutral or slightly acidic conditions (pH7.0). Enzyme analysis revealed laccase, NADH-DCIP reductase, and azoreductase as key contributors to MG decolorization, with significantly higher activity in mixed biofilms. Importantly, the bio-decolorization process transformed MG into non-phytotoxic compounds, demonstrated by seed germination and growth assays. These findings propose a promising and environmentally safe approach for MG bioremediation using mixed Pseudomonas biofilms.
本研究调查了由两种假单胞菌菌株（NAA22和NAA23）形成的混合生物膜对孔雀石绿（MG）染料的生物脱色应用。分离出的菌株表现出生物膜形成能力和MG脱色能力。与单个菌株相比，混合生物膜表现出显著更强的生物膜形成能力和MG脱色能力（94.3%），表明存在协同相互作用。这种脱色效率超过了先前报道的单菌株脱色值。混合生物膜能耐受较宽的温度范围（20 - 40°C）和pH范围（5 - 9），在中性或微酸性条件（pH7.0）下脱色效果最佳。酶分析表明，漆酶、NADH - DCIP还原酶和偶氮还原酶是MG脱色的关键因素，在混合生物膜中的活性显著更高。重要的是，种子发芽和生长试验表明，生物脱色过程将MG转化为非植物毒性化合物。这些发现为利用混合假单胞菌生物膜进行MG生物修复提出了一种有前景且环境安全的方法。
