Liu Chun, Udawatte Nadeeka S, Liaw Andrew, Staples Reuben, Salomon Carlos, Seneviratne Chaminda Jayampath, Ivanovski Sašo, Han Pingping
School of Dentistry, Center for Oral-facial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics nanodiagnostic and therapeutic group, The University of Queensland, Brisbane, QLD, 4006, Australia.
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, The University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Brisbane, QLD, 4029, Australia.
Adv Healthc Mater. 2025 Mar;14(6):e2403300. doi: 10.1002/adhm.202403300. Epub 2025 Jan 2.
With the advent of multi-layered and 3D scaffolds, the understanding of microbiome composition and pathogenic mechanisms within polymicrobial biofilms is continuously evolving. A fundamental component in mediating the microenvironment and bacterial-host communication within the biofilm are bilayered nanoparticles secreted by bacteria, known as bacterial extracellular vesicles (BEVs), which transport key biomolecules including proteins, nucleic acids, and metabolites. Their characteristics and microbiome profiles are yet to be explored in the context of in vitro salivary polymicrobial biofilm. This pilot study aimed to compare the profiles of BEVs from salivary biofilm cultured on a 2D tissue culture plate and 3D melt electrowritten medical-grade polycaprolactone (MEW mPCL) scaffold. BEVs derived from MEW mPCL biofilm exhibited enhanced purity and yield without altered EV morphology and lipopolysaccharide (LPS) content, with enriched BEVs-associated DNA from Capnocytophaga, porphyromonas, and veillonella genus. Moreover, compared to saliva controls, MEW mPCL BEVs showed comparable DNA expression of Tannerella forsythia, and Treponema denticola and significantly higher expression in Porphyromonas gingivalis, Eikenella corrodens and Lactobacillus acidophilus. Together, these findings highlight a more detailed microbial profile with BEVs derived from salivary biofilms cultured on 3D MEW PCL scaffolds, which facilitates an effective in vitro model with a greater resemblance to naturally occurring biofilms.
随着多层和3D支架的出现，对多微生物生物膜内微生物群组成和致病机制的理解也在不断发展。细菌分泌的双层纳米颗粒，即细菌细胞外囊泡（BEV），是介导生物膜内微环境和细菌与宿主通讯的一个基本组成部分，它能运输包括蛋白质、核酸和代谢物在内的关键生物分子。在体外唾液多微生物生物膜的背景下，它们的特征和微生物群概况还有待探索。这项初步研究旨在比较在二维组织培养板和三维熔纺电写医用级聚己内酯（MEW mPCL）支架上培养的唾液生物膜中BEV的概况。源自MEW mPCL生物膜的BEV表现出更高的纯度和产量，而其形态和脂多糖（LPS）含量未改变，且与二氧化碳嗜纤维菌属、卟啉单胞菌属和韦荣球菌属相关的BEV富集DNA。此外，与唾液对照相比，MEW mPCL BEV显示出与福赛坦纳菌和具核梭杆菌相当的DNA表达，而牙龈卟啉单胞菌、腐蚀艾肯菌和嗜酸乳杆菌的表达则显著更高。总之，这些发现突出了源自三维MEW PCL支架上培养的唾液生物膜的BEV更详细的微生物概况，这有助于建立一个与天然生物膜更相似的有效体外模型。
