Udawatte Nadeeka S, Liu Chun, Staples Reuben, Han Pingping, Kumar Purnima S, Arumugam Thiruma V, Ivanovski Sašo, Seneviratne Chaminda J
Center for Oral-facial Regeneration, Rehabilitation and Reconstruction (COR3), School of Dentistry, The University of Queensland, Brisbane, QLD 4006, Australia.
Department of Periodontics and Oral Medicine, School of Dentistry, The Ohio State University, Columbus, OH 43210, USA.
Int J Mol Sci. 2025 Jul 3;26(13):6403. doi: 10.3390/ijms26136403.
Three-dimensional (3D) scaffold systems have proven instrumental in advancing our understanding of polymicrobial biofilm dynamics and probiotic interactions within the oral environment. Among oral probiotics, K12 () has shown considerable promise in modulating microbial homeostasis; however, its long-term therapeutic benefits are contingent upon successful and sustained colonization of the oral mucosa. Despite its clinical relevance, the molecular mechanisms underlying the adhesion, persistence, and integration of Ssk12 into the native oral microbiome/biofilm remain inadequately characterized. In this pilot study, we explored the temporal colonization dynamics of and its impact on the structure and functional profiles of salivary-derived biofilms cultivated on melt-electrowritten poly(ε-caprolactone) (MEW-mPCL) scaffolds, which emulate the native oral niche. Colonization was monitored via fluorescence in situ hybridization (smFISH), confocal microscopy, and RT-qPCR, while shifts in community composition and function were assessed using 16S rRNA sequencing and meta-transcriptomics. A single administration of exhibited transient colonization lasting up to 7 days, with detectable presence diminishing by day 10. This was accompanied by short-term increases in and populations. Functional analyses revealed increased transcriptional signatures linked to oxidative stress resistance and metabolic adaptation. These findings suggest that even short-term probiotic colonization induces significant functional changes, underscoring the need for strategies to enhance probiotic persistence.
三维（3D）支架系统已被证明有助于推进我们对口腔环境中多微生物生物膜动态和益生菌相互作用的理解。在口腔益生菌中，K12（）在调节微生物稳态方面显示出相当大的前景；然而，其长期治疗益处取决于在口腔黏膜上成功且持续的定植。尽管其具有临床相关性，但Ssk12粘附、持续存在并整合到天然口腔微生物群/生物膜中的分子机制仍未得到充分表征。在这项初步研究中，我们探索了K12的时间定植动态及其对在模拟天然口腔生态位的熔喷电纺聚（ε-己内酯）（MEW-mPCL）支架上培养的唾液衍生生物膜的结构和功能谱的影响。通过荧光原位杂交（smFISH）、共聚焦显微镜和RT-qPCR监测定植情况，同时使用16S rRNA测序和宏转录组学评估群落组成和功能的变化。单次施用K12表现出持续长达7天的短暂定植，到第10天时可检测到的存在减少。这伴随着和种群的短期增加。功能分析揭示了与抗氧化应激和代谢适应相关的转录特征增加。这些发现表明，即使是短期的益生菌定植也会诱导显著的功能变化，强调了需要采取策略来增强益生菌的持久性。
