Okafor Izuchukwu Azuka, Mbagwu Smart Ikechukwu, Chidera Ikwuneme Gibson, Augustine Ezenduka Chiemeka, Anagboso Ikenna Makuachukwu, Onyema Chikwesiri Emmanuel
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, P.O. Box 5001, 435101 Nnewi, AN, Nigeria.
Molecular Diagnostics and Research Laboratory, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, P.O. Box 5001, 435101 Nnewi, AN, Nigeria.
Rev Int Androl. 2024 Dec;22(4):59-67. doi: 10.22514/j.androl.2024.031. Epub 2024 Dec 30.
Tramadol, an opioid analgesic, is known to induce testicular damage and impair reproductive parameters. Vitamin D3, recognized for its antioxidant and protective properties, might offer a potential protective effect against tramadol-induced testicular damage. This study observed the effects of co-administration of vitamin D3 and tramadol on serum kisspeptin levels, testicular histology, semen parameters, testosterone levels, and oxidative stress markers in male rats.
Fifteen male rats weighing between 150 and 250 g were randomly divided into three groups (n = 5 per group). Group A was the control, receiving only distilled water. Group B was administered 20 mg/kg body weight of tramadol daily, while group C received both 20 mg/kg body weight of tramadol and 25 μg/kg body weight of vitamin D3 daily. The treatments were administered orally for 14 days. Post-administration body weight, relative testicular weight, serum kisspeptin levels, semen parameters, testosterone levels and oxidative stress markers (catalase, glutathione and malonaldehyde) were measured. Testicular histology was also examined using photomicrography.
No significant differences were observed in body weights, relative testicular weights, serum kisspeptin levels, semen parameters, testosterone levels, or oxidative stress markers among the experimental groups ( > 0.05). Histological analysis in the tramadoltreated group exhibited significant degradation of spermatozoa, which was not mitigated by vitamin D3 co-administration compared to the control group.
The study demonstrates that vitamin D3 supplementation does not significantly ameliorate tramadol-induced testicular damage. There is a need for further research with varied doses and longer durations to further explore the potential protective mechanisms of vitamin D3.
曲马多是一种阿片类镇痛药，已知会导致睾丸损伤并损害生殖参数。维生素D3因其抗氧化和保护特性而闻名，可能对曲马多诱导的睾丸损伤具有潜在的保护作用。本研究观察了维生素D3与曲马多联合给药对雄性大鼠血清亲吻素水平、睾丸组织学、精液参数、睾酮水平和氧化应激标志物的影响。
将15只体重在150至250克之间的雄性大鼠随机分为三组（每组n = 5）。A组为对照组，仅接受蒸馏水。B组每天给予20毫克/千克体重的曲马多，而C组每天接受20毫克/千克体重的曲马多和25微克/千克体重的维生素D3。治疗经口服给药14天。给药后测量体重、相对睾丸重量、血清亲吻素水平、精液参数、睾酮水平和氧化应激标志物（过氧化氢酶、谷胱甘肽和丙二醛）。还使用显微镜摄影检查睾丸组织学。
各实验组之间在体重、相对睾丸重量、血清亲吻素水平、精液参数、睾酮水平或氧化应激标志物方面未观察到显著差异（P>0.05）。曲马多治疗组的组织学分析显示精子有明显退化，与对照组相比，联合给予维生素D3并未减轻这种退化。
该研究表明补充维生素D3并不能显著改善曲马多诱导的睾丸损伤。需要进一步研究不同剂量和更长疗程，以进一步探索维生素D3的潜在保护机制。
