Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Egypt.
Department of Pathology, Animal Reproductive Research Institute, Giza, 12556, Egypt.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Jun;397(6):4309-4319. doi: 10.1007/s00210-023-02874-y. Epub 2023 Dec 13.
The present study aimed to investigate the protective potential of naringin (NG) against di-n-butyl phthalate (DBP)- induced testicular damage and impairment of spermatogenesis in rats. Forty-two male Wistar albino rats were divided into six equal groups, and treated orally, 3 times weekly for 8 successive weeks. Control vehicle group was administrated olive oil, naringin-treated group was administered NG (80 mg/kg), DBP 250- and DBP 500- intoxicated groups received DBP (250 mg/kg) and (500 mg/kg), respectively, NG + DBP 250 and NG + DBP 500 groups received NG, an hour prior to DBP 250 and 500 administration. The results revealed that DBP induced dose-dependent male reproductive dysfunctions, included a significant decrease in the serum testosterone level concomitantly with significant decreases in the sperm count, viability, and total motility. Meanwhile, DBP significantly increased the testicular malondialdehyde level with significant reductions of glutathione content and catalase activity. Histopathologically, DBP provoked absence of spermatozoa, degenerative changes in the cell layers of seminiferous tubules and a significant decrease in the thickness of the seminiferous tubules epithelium. Conversely, the concomitant treatment with NG, one hour before DBP 250 or 500- intoxication mitigated the dose-dependent reproductive dysfunctions induced by DBP, evidenced by significant increases of serum testosterone level, sperm motility, count and viability along with marked improvement of the oxidant/antioxidant status and testicular histoarchitecture. In conclusion, the findings recorded herein proved that NG could mitigate DBP-induced testicular damage and impairment of spermatogenesis, suggesting the perspective of using NG as a natural protective and therapeutic agent for alleviating the reproductive dysfunctions and improving reproductive performance, mainly via its potent antioxidant activity.
本研究旨在探讨柚皮苷（NG）对邻苯二甲酸二丁酯（DBP）诱导的大鼠睾丸损伤和精子发生障碍的保护潜力。42 只雄性 Wistar 白化大鼠被分为六组，每周口服 3 次，连续 8 周。对照组给予橄榄油，柚皮苷处理组给予 NG（80mg/kg），DBP250 和 DBP500 染毒组分别给予 DBP（250mg/kg）和（500mg/kg），NG+DBP250 和 NG+DBP500 组在给予 DBP250 和 500 之前 1 小时给予 NG。结果表明，DBP 诱导的雄性生殖功能障碍呈剂量依赖性，表现为血清睾酮水平显著降低，精子计数、活力和总活力显著降低。同时，DBP 显著增加睾丸丙二醛水平，谷胱甘肽含量和过氧化氢酶活性显著降低。组织病理学检查显示，DBP 引起精子缺失，生精小管细胞层退行性改变，生精小管上皮厚度显著降低。相反，NG 与 DBP 同时给药，在 DBP250 或 500 染毒前 1 小时给药，可减轻 DBP 诱导的生殖功能障碍，表现为血清睾酮水平、精子活力、计数和活力显著增加，氧化应激/抗氧化状态和睾丸组织形态明显改善。总之，本研究结果证明，NG 可减轻 DBP 诱导的睾丸损伤和精子发生障碍，表明 NG 作为一种天然保护和治疗剂，可用于缓解生殖功能障碍和提高生殖性能，主要通过其强大的抗氧化活性。
