Anbazhagan Sowmya, Krishnan E Arvindh, S Divya, Sureshkumar Mathavi
Microbiology, Vinayaka Mission's Kirupananda Variyar Medical College and Hospital, Salem, IND.
Medical Microbiology, Vinayaka Mission's Kirupananda Variyar Medical College and Hospital, Salem, IND.
Cureus. 2024 Dec 3;16(12):e75032. doi: 10.7759/cureus.75032. eCollection 2024 Dec.
Introduction The antimicrobial resistance of is variable and is influenced by both geographic location and regional antibiotic use. The overuse of antibiotics, especially in hospitalised patients, suppresses the growth and persistence of drug-resistant bacteria. This study aimed to detect the prevalence of carbapenem-resistant and the genes responsible for the resistance. Methods A cross-sectional study has been conducted over the course of two years, from October 2021 to September 2023. A total of 2,152 samples, including pus, blood, urine, sputum and various body fluids, were collected and subjected to study. All data were analysed and presented as frequency with percentage. Results Out of 2,152 samples, 659 (32.1%) samples showed growth. Among them, 250 (38%) were found to be , of which 22 (8.8%) were resistant to carbapenems. The isolates were (nine, 40%) followed by (eight, 36%), (two, 9%), (one, 5%), (1, 5%), and (1, 5%). NDM (14, 63.63%) was the most common gene detected from the isolates. Conclusion Our research leads us to the conclusion that resistance to carbapenem medication can result from either the generation of carbapenemase or from non-carbapenemase mechanisms like loss of porin channels or an increase in the efflux pump. According to our research, the primary source of carbapenem resistance is metallo-β-lactamase. Therefore, it is critical for all the laboratories to identify the mechanism and incidence of carbapenem resistance in order to support epidemiological research, infection control and antibiotic stewardship.
引言 [细菌名称]的抗菌耐药性具有变异性，且受地理位置和区域抗生素使用情况的影响。抗生素的过度使用，尤其是在住院患者中，会抑制耐药菌的生长和持续存在。本研究旨在检测耐碳青霉烯类[细菌名称]的流行情况及其耐药相关基因。
方法 在2021年10月至2023年9月的两年时间里进行了一项横断面研究。共收集了2152份样本，包括脓液、血液、尿液、痰液和各种体液，并进行研究。所有数据均进行分析，并以频率和百分比形式呈现。
结果 在2152份样本中，659份（32.1%）样本有细菌生长。其中，250份（38%）被鉴定为[细菌名称]，其中22份（8.8%）对碳青霉烯类耐药。分离菌株以[具体细菌种类1]（9株，40%）居多，其次是[具体细菌种类2]（8株，36%）、[具体细菌种类3]（2株，9%）、[具体细菌种类4]（1株，5%）、[具体细菌种类5]（1株，5%）和[具体细菌种类6]（1株，5%）。NDM（14株，63.63%）是从分离菌株中检测到的最常见基因。
结论 我们的研究得出结论，对碳青霉烯类药物的耐药性可能源于碳青霉烯酶的产生，也可能源于非碳青霉烯酶机制，如孔蛋白通道的丧失或外排泵的增加。根据我们的研究，碳青霉烯耐药的主要来源是金属β-内酰胺酶。因此，所有实验室确定碳青霉烯耐药的机制和发生率对于支持流行病学研究、感染控制和抗生素管理至关重要。
