Hyder Don, Miller Eric, Rankin Stephen, Turner Danielle, Rogelj Snezna, Tello-Aburto Rodolfo, Smiley Desiree, Baker Bryden, Vandeever Holly, Esmiol Hunter, Begay Russell, Barajas Jonathan, Martinez Sergio
San Juan College, Farmington, NM, USA.
Pinon Family Practice, Farmington, NM, USA.
N M J Sci. 2023;57:50-63.
A study was initiated during the summers of 2015-2019 to characterize the floral and chemical components in a local honey (clinical honey) that was being used in a Food and Drug Administration (FDA) approved clinical study designed to evaluate effectiveness in controlling topical community acquired methicillin resistant (caMRSA) infections. Floral sources were determined by collecting nectar and pollen from plants visited by bees within the area where the local honey is being produced (Study Area). Pollen characteristics were determined by using both light microscopy (LM) and scanning electron microscopy (SEM). This information was compared to pollen collected by a pollen trap on hives within the study area. The nectars and the medical honey were analyzed for biologically active compounds using Solid Phase Micro Extraction (SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). This equipment allows a researcher to extract, separate, and identify chemical components of a honey or nectar sample. Fourteen biologically active compounds were identified from the eighteen floral sources and the clinical honey. Nine of the fourteen compounds were selected for standard minimum inhibitory concentration (MIC) antibacterial assay using CAMRSA ATCC BAA-44 strain. Phenylethyl alcohol and phenylacetaldehyde were the only compounds exhibiting promising activity against caMRSA. Both exhibited bacteriostatic activity. A variety of antibiotic compounds were unique to clinical honey. This suggests that the various nectars provide a large chemical base for antibiotic compounds.
2015年至2019年夏季开展了一项研究，旨在对一种当地蜂蜜（临床蜂蜜）的花卉和化学成分进行表征，该蜂蜜正在用于美国食品药品监督管理局（FDA）批准的一项临床研究，以评估其控制局部社区获得性耐甲氧西林金黄色葡萄球菌（caMRSA）感染的有效性。通过从当地蜂蜜生产区域（研究区域）内蜜蜂采蜜的植物上采集花蜜和花粉来确定花卉来源。通过光学显微镜（LM）和扫描电子显微镜（SEM）确定花粉特征。将这些信息与研究区域内蜂箱上的花粉采集器收集的花粉进行比较。使用固相微萃取（SPME）和气相色谱 - 质谱联用仪（GC - MS）对花蜜和医用蜂蜜中的生物活性化合物进行分析。该设备使研究人员能够提取、分离和鉴定蜂蜜或花蜜样品的化学成分。从18种花卉来源和临床蜂蜜中鉴定出14种生物活性化合物。选择其中9种化合物，使用CAMRSA ATCC BAA - 44菌株进行标准最小抑菌浓度（MIC）抗菌试验。苯乙醇和苯乙醛是仅有的对caMRSA表现出有前景活性的化合物。两者均表现出抑菌活性。临床蜂蜜含有多种独特的抗生素化合物。这表明各种花蜜为抗生素化合物提供了大量的化学基础。
