Li Letian, Wang Mengjiao, Dong Yongzhen, Yu Deyang, Chen Yiping
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034 Liaoning, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058 Zhejiang, China.
ACS Nano. 2025 Jan 14;19(1):920-932. doi: 10.1021/acsnano.4c12394. Epub 2025 Jan 3.
Development of a multiplexed and sensitive biosensing platform is a priority for public health security. We report a micropore resistance counting platform based on polystyrene microsphere size-based encoding and Argonaute (CbAgo) decoding for multiplexed and ultrasensitive detection. Initially, we constructed a target DNA-modified polystyrene microsphere coding system based on micropore resistance counting. Subsequently, the precise recognition and cleavage capabilities of the guide DNA-activated CbAgo protein enable the decoding of the encoded microsphere system. Changes in the concentration of polystyrene microspheres are presented as a signal readout. The platform demonstrated excellent performance in multiplexed detection of three mycotoxins (with a sensitivity range over 4 orders of magnitude reaching the pg/mL level) and two inflammatory markers at pg/mL. Combining precise enzyme cleavage by CbAgo with micropore resistance counting, the developed platform is a multiplexed and highly sensitive detection tool with wide-ranging potential in applications such as clinical diagnosis, food safety inspection, and environmental monitoring.
开发一个多重且灵敏的生物传感平台是公共卫生安全的首要任务。我们报告了一种基于聚苯乙烯微球尺寸编码和Argonaute（CbAgo）解码的微孔电阻计数平台，用于多重和超灵敏检测。最初，我们基于微孔电阻计数构建了一个目标DNA修饰的聚苯乙烯微球编码系统。随后，引导DNA激活的CbAgo蛋白的精确识别和切割能力实现了对编码微球系统的解码。聚苯乙烯微球浓度的变化作为信号读出。该平台在三种霉菌毒素（灵敏度范围超过4个数量级，达到pg/mL水平）和两种pg/mL水平的炎症标志物的多重检测中表现出优异性能。将CbAgo的精确酶切与微孔电阻计数相结合，所开发的平台是一种多重且高度灵敏的检测工具，在临床诊断、食品安全检测和环境监测等应用中具有广泛的潜在应用价值。
