Wang Jingwen, Liu Wenxiu, Liu Zhenshuo, Yu Xiaodong, Zhang Hongyan, Du Shuyuan
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong, College of Life Science, Normal University, Shandong Normal University, Jinan, 250014, People's Republic of China.
Rongcheng Market Supervision and Administration Comprehensive Service Center, Weihai, 264300, People's Republic of China.
Mikrochim Acta. 2025 Jan 3;192(1):52. doi: 10.1007/s00604-024-06917-w.
A composite nanomaterial of Prussian blue@gold nanoparticles (PB@Au) with catalytic and photothermal properties was proposed, which combined with anti-matrix interference aptamers to achieve robust specificity and sensitivity in the detection of Salmonella typhimurium (S. typhimurium). The detection probe, PB@Au-Aptamer (PB@Au-Apt), was designed to exhibit high specificity for the target and catalyze the signal generation to produce a color change, thereby enabling rapid detection. Additionally, the excellent photothermal performance of the PB@Au catalytic system was utilized for multimodal sensitive detection in the multimodal nanoenzyme-linked aptamer assay. Moreover, the utilization of both catalytic and photothermal dual-mode detection was mutually verified to enhance detection accuracy. Under optimal conditions, the detection of S. typhimurium in a sample can be completed in 2 h. The developed assay exhibited exceptional specificity in detecting S. typhimurium, with an impressive detection limit down to 23 CFU·mL. Furthermore, the assay exhibited excellent repeatability and stability. Real sample analyses have proven the high reliability and practicality of this assay, highlighting its significant potential for applications in food safety testing.
提出了一种具有催化和光热性能的普鲁士蓝@金纳米颗粒复合纳米材料（PB@Au），其与抗基质干扰适配体相结合，在鼠伤寒沙门氏菌（S. typhimurium）检测中实现了强大的特异性和灵敏度。检测探针PB@Au-适配体（PB@Au-Apt）被设计成对目标具有高特异性，并催化信号产生以产生颜色变化，从而实现快速检测。此外，PB@Au催化系统优异 的光热性能被用于多模式纳米酶联适配体分析中的多模式灵敏检测。而且，催化和光热双模式检测的运用相互验证，以提高检测准确性。在最佳条件下，样品中鼠伤寒沙门氏菌的检测可在2小时内完成。所开发的分析方法在检测鼠伤寒沙门氏菌时表现出卓越的特异性，检测限低至23 CFU·mL，令人印象深刻。此外，该分析方法具有出色的重复性和稳定性。实际样品分析证明了该分析方法的高可靠性和实用性，突出了其在食品安全检测中的巨大应用潜力。
