Lu Zhang, Liu Wenxiu, Cai Yun, Zhao Tao, Cui Mengqi, Zhang Hongyan, Du Shuyuan
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China.
School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
Food Chem. 2022 Aug 15;385:132649. doi: 10.1016/j.foodchem.2022.132649. Epub 2022 Mar 7.
This work reports a sensitive and accurate multimode detection method to detect Salmonella typhimurium using inherent color, photothermal and catalytic properties of Prussian blue@gold nanoparticles (PB@Au). The inherent color of PB@Au can realize direct visual detection while the temperature increase (ΔT) of it can realize sensitive and quantitative photothermal detection. Moreover, catalytic coloration detection is applied to further amplify detection signal. The risk limit, prevention and control of Salmonella typhimurium can be more intuitively displayed through catalytic color overlap degree between PB@Au and catalytic product. The sensitivity of method is improved through photothermal and catalytic coloration detection (10 CFU·mL) compared with direct visual detection (10 CFU·mL). The multimode detection improves the accuracy of method, and exhibits good repeatability, acceptable selectivity and stability. This method is also successfully applied in real samples, displaying its good practical applicability.
这项工作报道了一种灵敏且准确的多模式检测方法,该方法利用普鲁士蓝@金纳米颗粒(PB@Au)的固有颜色、光热和催化特性来检测鼠伤寒沙门氏菌。PB@Au的固有颜色可实现直接视觉检测,而其温度升高(ΔT)可实现灵敏且定量的光热检测。此外,催化显色检测用于进一步放大检测信号。通过PB@Au与催化产物之间的催化颜色重叠程度,可更直观地展示鼠伤寒沙门氏菌的风险限度、预防和控制情况。与直接视觉检测(10⁵ CFU·mL⁻¹)相比,通过光热和催化显色检测(10³ CFU·mL⁻¹)提高了该方法的灵敏度。多模式检测提高了该方法的准确性,并表现出良好的重复性、可接受的选择性和稳定性。该方法也成功应用于实际样品,显示出其良好的实际适用性。