Dai Huasong, Zhang Yingyue, Zhao Wenshi, Guo Rui, Qian Sihan, Xu Yang, Li Yuxuan, Liu Yang, Liu Hongbo
Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, PR China.
Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, PR China.
Anal Chim Acta. 2025 Feb 8;1338:343589. doi: 10.1016/j.aca.2024.343589. Epub 2024 Dec 25.
The foodborne pathogens, e.g., Salmonella typhimurium (S. typ) and Staphylococcus aureus (S. aureus), pose a serious threat to human health. Accurate identification, rapid detection and efficient inactivation are crucial in the early diagnosis and treatment of S. typ and S. aureus. To date, however, the majority of studies have only concentrated on the construction of single-function biological platform for detection or inactivation of S. typ and S. aureus. Therefore, it is imperative to develop a multifunctional surface-enhanced Raman scattering (SERS) biosensor that can effectively sterilize S. typ and S. aureus while simultaneously achieving sensitive detection and selective identification.
Herein, we designed and constructed a multifunctional SERS biosensor based on sandwich structure of "capture probe/bacteria/signal probe" in order to simultaneously identify, detect and kill S. typ and S. aureus. Aptamer-modified ZnO/Ag was used as a capture probe to accurately identify and capture the target bacteria in complex environments. Au@Ag-4-MPBA-Aptamer was employed as signal probe to provide the corresponding bacterial SERS "fingerprint" information. The SERS enhancement mechanism of the sandwich-structure ZnO/Ag-Au@Ag SERS substrate was discussed. The sandwich-type SERS biosensor exhibited the strong localized surface plasmon resonance (LSPR) effect and the detection limit for S. typ and S. aureus was as low as 10 cfu/mL. Furthermore, the sandwich-type SERS biosensor offered excellent photothermal conversion efficiency (54.32 %), enabling photothermal killing of target bacteria when exposed to laser irradiation.
A dual enhancement strategy based on a sandwich structure was proposed to maximize the sensitivity of SERS signals using synergistic action of electromagnetic enhancement and chemical enhancement. SERS enhancement factor (EF) was as high as 4.67 × 10. In addition, the sandwich-type SERS biosensor not only exhibited negligible cytotoxicity, but also was proved to be a promising tool for photothermally inactivate of S. typ and S. aureus in food samples.
食源性病原体，如鼠伤寒沙门氏菌（S. typ）和金黄色葡萄球菌（S. aureus），对人类健康构成严重威胁。准确识别、快速检测和有效灭活对于S. typ和S. aureus的早期诊断和治疗至关重要。然而，迄今为止，大多数研究仅集中于构建用于检测或灭活S. typ和S. aureus的单功能生物平台。因此，开发一种多功能表面增强拉曼散射（SERS）生物传感器迫在眉睫，该传感器能够有效杀灭S. typ和S. aureus，同时实现灵敏检测和选择性识别。
在此，我们基于“捕获探针/细菌/信号探针”的三明治结构设计并构建了一种多功能SERS生物传感器，以同时识别、检测和杀灭S. typ和S. aureus。适配体修饰的ZnO/Ag用作捕获探针，以在复杂环境中准确识别和捕获目标细菌。Au@Ag-4-MPBA-适配体用作信号探针，以提供相应的细菌SERS“指纹”信息。讨论了三明治结构ZnO/Ag-Au@Ag SERS基底的SERS增强机制。三明治型SERS生物传感器表现出强烈的局域表面等离子体共振（LSPR）效应，对S. typ和S. aureus的检测限低至10 cfu/mL。此外，三明治型SERS生物传感器具有优异的光热转换效率（54.32%），在激光照射下能够对目标细菌进行光热杀灭。
提出了一种基于三明治结构的双重增强策略，利用电磁增强和化学增强的协同作用最大化SERS信号的灵敏度。SERS增强因子（EF）高达4.67×10。此外，三明治型SERS生物传感器不仅表现出可忽略不计的细胞毒性，而且被证明是用于食品样品中S. typ和S. aureus光热灭活的有前景的工具。
