Margońska Hanna B, Kozieradzka-Kiszkurno Małgorzata, Brzezicka Emilia, Haliński Łukasz P, Davies Kevin L
Department of Plant Taxonomy and Nature Conservation, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.
Department of Plant Experimental Biology and Biotechnology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.
Sci Rep. 2025 Jan 3;15(1):723. doi: 10.1038/s41598-024-84538-2.
Dienia is a small, pantropical genus of epidendroid Malaxideae orchids. The floral lip is upwardly directed and does not serve as a landing platform for pollinators. This role has been assumed by sepals and/or gynostemium or whole inflorescence. Information about the pollination and floral morphology of this genus is scarce. Field observations have revealed that the flowers are visited by small flies, midges, other small dipterans, ants, and mites etc. Preliminary observations have revealed that small liquid droplets of at least two types occur on the morphologically adaxial lip surface of Dienia: namely, simple secretions produced by epidermal cells, and cell sap released on the rupturing of raphide cells. These secretions were subjected to sequential organic solvent extraction and gas chromatography-mass spectrometry. Floral structures were investigated by means of scanning and transmission electron microscopy, and subjected to histochemical tests. The form of liquid droplets on the lip, as well as the presence of a food reward and the sequence of raphide development are reported here for the first time. Tree different form of calcium oxalate crystals were raported in Dienia ophrydis flowers.
迪尼亚兰属是附生的沼兰亚族中一个小型的泛热带属兰花。花唇向上，不作为传粉者的着陆平台。这个角色由萼片和/或蕊柱或整个花序承担。关于该属植物的授粉和花形态的信息很少。野外观察发现，该属植物的花朵会被小型苍蝇、蠓、其他小型双翅目昆虫、蚂蚁和螨虫等访花。初步观察发现，迪尼亚兰属植物形态上的近轴唇表面存在至少两种类型的小液滴：即表皮细胞产生的简单分泌物，以及针晶细胞破裂时释放的细胞液。对这些分泌物进行了连续的有机溶剂萃取和气相色谱 - 质谱分析。通过扫描电子显微镜和透射电子显微镜研究了花的结构，并进行了组织化学测试。本文首次报道了唇上液滴的形态、食物报酬的存在以及针晶发育的顺序。在长瓣迪尼亚兰的花朵中发现了三种不同形态的草酸钙晶体。
