Wiśniewska Natalia, Kowalkowska Agnieszka K, Kozieradzka-Kiszkurno Małgorzata, Krawczyńska Agnieszka T, Bohdanowicz Jerzy
Department of Plant Cytology and Embryology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.
Faculty of Materials Science and Engineering, Warsaw University of Technology, Wołoska 141, 02-507, Warsaw, Poland.
Protoplasma. 2018 Mar;255(2):485-499. doi: 10.1007/s00709-017-1156-2. Epub 2017 Sep 14.
Two representatives of section Lepidorhiza, previously sometimes considered conspecific, Bulbophyllum levanae and Bulbophyllum nymphopolitanum, demonstrated both similarities and differences in floral features. There were significant differences in the length of sepals and micromorphological features of the labellum. In both species, osmophores are located on the extended apices of sepals and possibly on petals. An abundance of proteins in tepals is probably associated with the unpleasant scent of the flowers, whereas the thin wax layers on the epidermis are probably involved in the maintenance of the brilliance of floral tepals, which strongly attracts flies. In all tepals of both species, we noted the presence of dihydroxyphenolic globules in the cytoplasm after staining with FeCl. Comparison with ultrastructure results revealed that they were associated with plastids containing plastoglobuli. The most remarkable feature was the presence of a prominent periplasmic space in the epidermal cells of both investigated species. Furthermore, in the labellum of B. levanae, the cuticle contained microchannels. The combination of periplasmic space and microchannels has not previously been recorded.
Lepidorhiza亚组的两个代表物种，即之前有时被认为是同种的莱瓦纳石豆兰（Bulbophyllum levanae）和仙女石豆兰（Bulbophyllum nymphopolitanum），在花部特征上既有相似之处，也有不同之处。萼片长度和唇瓣微观形态特征存在显著差异。在这两个物种中，发香体位于萼片的延长顶端，也可能位于花瓣上。花被片中丰富的蛋白质可能与花朵的难闻气味有关，而表皮上薄薄的蜡层可能与维持花被片的光泽有关，这能强烈吸引苍蝇。在用FeCl染色后，我们在两个物种的所有花被片中都注意到细胞质中存在二羟基酚小球。与超微结构结果对比发现，它们与含有质体小球的质体有关。最显著的特征是在两个被研究物种的表皮细胞中都存在一个明显的周质空间。此外，在莱瓦纳石豆兰的唇瓣中，角质层含有微通道。周质空间和微通道的组合此前尚未有记录。
