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RNA结合蛋白DAZAP1通过抑制铁死亡促进胰腺癌进展。

RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis.

作者信息

Wang Xinqing, Fan Hao, Ye Xiaoping, Hu Yu, Xiao Yan, Zhang Ming, Xu Yonghui, Song Jianjun, Luo Yongyun

机构信息

Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Shengli Street, Xingqing District, Ningxia Hui Autonomous Region 804, Yinchuan City, 753400, China.

School of Clinical Medicine, Ningxia Medical University, Yinchuan City, China.

出版信息

Eur J Med Res. 2025 Jan 4;30(1):3. doi: 10.1186/s40001-024-02261-0.

DOI:10.1186/s40001-024-02261-0
PMID:39754243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11699656/
Abstract

BACKGROUND

Pancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options.

OBJECTIVES

This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for its diagnosis and treatment.

METHODS

DAZAP1 expression in PC tissues, normal tissues and cell lines was assessed using immunohistochemistry (IHC), reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. DAZAP1 knockdown was achieved through plasmid transfection, and its effects on ferroptosis and PC progression were evaluated using RT-qPCR, western blotting, CCK-8 assays, EdU staining, Fe content measurement, reactive oxygen species (ROS) detection, wound healing and Transwell migration assays.

RESULTS

DAZAP1 expression was significantly upregulated in PC tissues and cell lines compared to normal counterparts. DAZAP1 knockdown suppressed PC cell proliferation and induced ferroptosis, while ferroptosis inhibition reversed these effects, enhancing PC cell proliferation and metastasis.

CONCLUSIONS

DAZAP1 suppression promotes ferroptosis, thereby inhibiting PC cell proliferation and metastasis. These findings suggest that DAZAP1 is a potential therapeutic target for PC.

摘要

背景

胰腺癌（PC）是一种侵袭性很强的恶性肿瘤，由于其晚期诊断和有限的治疗选择，预后较差。

目的

本研究旨在阐明胰腺癌进展的分子机制，并确定其诊断和治疗的潜在分子靶点。

方法

采用免疫组织化学（IHC）、逆转录定量聚合酶链反应（RT-qPCR）和蛋白质印迹法评估DAZAP1在胰腺癌组织、正常组织和细胞系中的表达。通过质粒转染实现DAZAP1基因敲低，并使用RT-qPCR、蛋白质印迹法、CCK-8法、EdU染色、铁含量测定、活性氧（ROS）检测、伤口愈合和Transwell迁移试验评估其对铁死亡和胰腺癌进展的影响。

结果

与正常组织相比，DAZAP1在胰腺癌组织和细胞系中的表达显著上调。DAZAP1基因敲低抑制了胰腺癌细胞的增殖并诱导了铁死亡，而铁死亡抑制则逆转了这些作用，增强了胰腺癌细胞的增殖和转移。

结论

抑制DAZAP1可促进铁死亡，从而抑制胰腺癌细胞的增殖和转移。这些发现表明DAZAP1是胰腺癌的一个潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/db13e6fd8bf2/40001_2024_2261_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/09acbb56a4a5/40001_2024_2261_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/f45895546ee0/40001_2024_2261_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/7627b95d4848/40001_2024_2261_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/dcd1684a7aa8/40001_2024_2261_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/65a57fccb436/40001_2024_2261_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/db13e6fd8bf2/40001_2024_2261_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/09acbb56a4a5/40001_2024_2261_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/f45895546ee0/40001_2024_2261_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/7627b95d4848/40001_2024_2261_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/dcd1684a7aa8/40001_2024_2261_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/65a57fccb436/40001_2024_2261_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdc/11699656/db13e6fd8bf2/40001_2024_2261_Fig6_HTML.jpg

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本文引用的文献

1
The Molecular and Biological Function of MEF2D in Leukemia.MEF2D 在白血病中的分子和生物学功能。
Adv Exp Med Biol. 2024;1459:379-403. doi: 10.1007/978-3-031-62731-6_17.
2
Necrostatin-1: a promising compound for neurological disorders.坏死抑制因子-1：一种治疗神经疾病的有前景的化合物。
Front Cell Neurosci. 2024 Jun 27;18:1408364. doi: 10.3389/fncel.2024.1408364. eCollection 2024.
3
Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1.细胞坏死性凋亡导致脱氧雪腐镰刀菌烯醇的肠道毒性，并由甲基转移酶 SETDB1 介导。
J Hazard Mater. 2024 Aug 5;474:134601. doi: 10.1016/j.jhazmat.2024.134601. Epub 2024 May 12.
4
[Cancer incidence and mortality in China, 2022].[2022年中国癌症发病率和死亡率]
Zhonghua Zhong Liu Za Zhi. 2024 Mar 23;46(3):221-231. doi: 10.3760/cma.j.cn112152-20240119-00035.
5
Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis.外泌体分析 Glypican 1 mRNA 和蛋白在胰腺癌诊断和预后中的作用。
Adv Sci (Weinh). 2024 Mar;11(11):e2306373. doi: 10.1002/advs.202306373. Epub 2024 Jan 10.
6
MiR-320a upregulation improves IL-1β-induced osteoarthritis via targeting the DAZAP1 and MAPK pathways.miR-320a 的上调通过靶向 DAZAP1 和 MAPK 通路改善了 IL-1β 诱导的骨关节炎。
J Orthop Surg Res. 2023 Jul 28;18(1):541. doi: 10.1186/s13018-023-03984-2.
7
Identification of ferroptosis-associated biomarkers in Stanford type A aortic dissection based on machine learning.基于机器学习的 Stanford A 型主动脉夹层中与铁死亡相关生物标志物的鉴定
Am J Transl Res. 2023 May 15;15(5):3092-3114. eCollection 2023.
8
Pancreatic Cancer: Changing Epidemiology and New Approaches to Risk Assessment, Early Detection, and Prevention.胰腺癌：流行病学变化及风险评估、早期检测和预防的新方法。
Gastroenterology. 2023 Apr;164(5):752-765. doi: 10.1053/j.gastro.2023.02.012. Epub 2023 Feb 18.
9
DAZAP1 facilitates the alternative splicing of KITLG to promote multiple myeloma cell proliferation via ERK signaling pathway.DAZAP1 通过 ERK 信号通路促进 KITLG 的可变剪接，从而促进多发性骨髓瘤细胞增殖。
Aging (Albany NY). 2022 Oct 13;14(19):7972-7985. doi: 10.18632/aging.204326.
10
Personalized pancreatic cancer therapy: from the perspective of mRNA vaccine.个性化胰腺癌治疗：从 mRNA 疫苗的角度。
Mil Med Res. 2022 Oct 13;9(1):53. doi: 10.1186/s40779-022-00416-w.