Zhao Wan-Ting, Herrmann Karl-Heinz, Wei Weiwei, Krämer Martin, Dahmen Uta, Reichenbach Jürgen R
Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany.
Department of General, Visceral and Vascular Surgery, Experimental Transplantation Surgery, Jena University Hospital, Jena, Germany.
MAGMA. 2025 Jan 4. doi: 10.1007/s10334-024-01223-1.
To establish an arterial spin labeling (ASL) protocol for rat livers that improves data reliability and reproducibility for perfusion quantification.
This study used respiratory-gated, single-slice, FAIR-based ASL imaging with multiple inversion times (TI) in rat livers. Quality assurance measures included: (1) introduction of mechanical ventilation to ensure consistent respiratory cycles by controlling the respiratory rate (45 bpm), tidal volume (10 ml/kg), and inspiration: expiration ratio (I:E ratio, 1:2), (2) optimization of the trigger window for consistent trigger points, and (3) use of fit residual map and coefficient of variance as metrics to assess data quality. We compared image quality, perfusion maps, and fit residual maps between mechanically ventilated and non-ventilated animals, as well as repeated ASL measurements (session = 4 per animal) in two mechanically ventilated animals.
Perfusion measurements over multiple sessions in mechanically ventilated rats exhibited low perfusion data variability and high reproducibility both within and between liver lobes. Image quality and perfusion maps were significantly improved in mechanically ventilated animals compared to non-ventilated animals.
The implementation of mechanical ventilation and optimized quality assurance protocols enhanced the reliability and reproducibility of FAIR-based multi-TI-ASL imaging in rat livers. Our findings demonstrate these measures as a robust approach for achieving consistent liver perfusion quantification in preclinical settings.
建立一种用于大鼠肝脏的动脉自旋标记（ASL）方案，以提高灌注定量数据的可靠性和可重复性。
本研究在大鼠肝脏中采用基于FAIR的多反转时间（TI）呼吸门控单层面ASL成像。质量保证措施包括：（1）引入机械通气，通过控制呼吸频率（45次/分钟）、潮气量（10毫升/千克）和吸气：呼气比（I:E比，1:2）确保一致的呼吸周期；（2）优化触发窗口以获得一致的触发点；（3）使用拟合残差图和变异系数作为评估数据质量的指标。我们比较了机械通气和非通气动物之间的图像质量、灌注图和拟合残差图，以及两只机械通气动物的重复ASL测量（每只动物4次测量）。
在机械通气的大鼠中，多组测量的灌注数据在肝叶内和肝叶间均表现出低灌注数据变异性和高可重复性。与非通气动物相比，机械通气动物的图像质量和灌注图有显著改善。
机械通气和优化的质量保证方案的实施提高了基于FAIR的多TI-ASL成像在大鼠肝脏中的可靠性和可重复性。我们的研究结果表明，这些措施是在临床前环境中实现一致的肝脏灌注定量的可靠方法。
