Serine-modified silver nanoparticle porous spray membrane: A novel approach to wound infection prevention and inflammation reduction.

Traditional wound care preparations frequently face challenges such as complex care protocols, poor patient compliance, limited skin permeability, lack of aesthetics, and inconvenience, in addition to the risk of bacterial infection. We developed a spray film preparation containing nanocellulose and L-serine modified nanosilver, capable of rapidly forming a transparent film on the skin within minutes of application. The incorporation of nanocellulose imparted protective, moisturizing, and breathable properties to the film, allowing for easy removal after use. Moreover, our innovative application of L-serine as a precursor for synthesizing silver nanoparticles not only overcomed the limitations of current silver nanoparticle synthesis methods but also enhanced antibacterial efficacy by leveraging the inherent antimicrobial properties and bacterial targeting capability of L-serine. Specifically, the addition of nanocellulose to the spray film forming system enhanced air permeability and drug carrying capacity by creating a lightweight porous structure, while also ensuring the antimicrobial and anti-inflammatory efficacy of serine modified silver nanoparticles. Mice treated with spray film formulations containing nanocellulose and silver nanoparticles demonstrated superior wound healing, normalization of keratin thickness, increased hair follicle count, and reduced inflammatory markers. In conclusion, our study developed a spray film that integrated excellent antimicrobial properties with user convenience. This research presented innovative strategies for clinical wound care, antibacterial infection management, and post laser cosmetic treatment, with the potential to substantially enhance wound care in practical application.