Cutarelli Anna, De Falco Francesca, Serpe Francesco, Izzo Simona, Fusco Giovanna, Catoi Cornel, Roperto Sante
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.
Dipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Naples, Italy.
Sci Rep. 2025 Jan 4;15(1):769. doi: 10.1038/s41598-024-81682-7.
BPV1, BPV2, BPV13, and BPV14 are all genotypes of bovine delta papillomaviruses (δPV), of which the first three cause infections in horses and are associated with equine sarcoids. However, BPV14 infection has never been reported in equine species. In this study, we examined 58 fresh and thawed commercial semen samples from healthy stallions. In 34 (58.6%), bovine δPV DNA was detected and quantified using droplet digital polymerase chain reaction (ddPCR). Real time quantitative PCR (qPCR) was able to identify bovine δPV DNA in 5 samples (8.6%). Of the BPV-infected semen samples, 15 were positive for BPV2 (~ 44.1%) on ddPCR and 4 (~ 11.7%) on qPCR; 12 (~ 35.3%) for BPV14 on ddPCR and 1 (~ 3%) by qPCR; 4 (~ 11.7%) for BPV1 on ddPCR, whereas qPCR failed to reveal this infection; 3 (~ 8.8%) for BPV13 on ddPCR; and BPV13 infection was not detected by qPCR. Our study showed for the first time that BPV14 is an additional infectious agent potentially responsible for infection in horses, as its transcripts were detected and quantified in some semen samples. Large-scale BPV14 screening is necessary to provide substantial data on the molecular epidemiology for a better understanding of the geographical divergence of BPV14 prevalence in different areas and how widespread BPV14 is among equids.
BPV1、BPV2、BPV13和BPV14均为牛δ乳头瘤病毒（δPV）的基因型，其中前三种可感染马匹并与马属肉瘤有关。然而，从未有过BPV14感染马属动物的报道。在本研究中，我们检测了58份来自健康种公马的新鲜和解冻后的商业精液样本。通过滴液数字聚合酶链反应（ddPCR）检测并定量了34份样本（58.6%）中的牛δPV DNA。实时定量PCR（qPCR）在5份样本（8.6%）中检测到了牛δPV DNA。在感染BPV的精液样本中，ddPCR检测显示15份样本BPV2呈阳性（约44.1%），qPCR检测显示4份样本呈阳性（约11.7%）；ddPCR检测显示12份样本（约35.3%）BPV14呈阳性，qPCR检测显示1份样本（约3%）呈阳性；ddPCR检测显示4份样本（约11.7%）BPV1呈阳性，而qPCR未能检测到该感染；ddPCR检测显示3份样本（约8.8%）BPV13呈阳性，qPCR未检测到BPV13感染。我们的研究首次表明，BPV14是另一种可能导致马匹感染的病原体，因为在一些精液样本中检测到了其转录本并进行了定量。有必要进行大规模的BPV14筛查，以提供关于分子流行病学的大量数据，从而更好地了解BPV14在不同地区流行情况的地理差异以及BPV14在马科动物中的传播范围。
