Shi Zhongliang, Jiao Yanna, Lai Zhizhen, Liu Juan, Yang Bo, Hu Mahong, Meng Jianbiao
Department of Critical Care Medicine, Tongde Hospital of Zhejiang Province, #234 Gucui Road, Hangzhou, 310012, Zhejiang, People's Republic of China.
Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, 310012, Zhejiang, People's Republic of China.
Sci Rep. 2025 Jan 4;15(1):828. doi: 10.1038/s41598-025-85148-2.
The intestinal barrier function is a critical defense mechanism in the human body, serving as both the primary target and initiating organ in cases of sepsis. Preserving the integrity of this barrier is essential for preventing complications and diseases, including sepsis and mortality. Despite this importance, the impact of resveratrol on intestinal barrier function remains unclear. Thus, this study aims to explore the potential beneficial effects of resveratrol on maintaining intestinal barrier function. Fifteen male Sprague Dawley rats, weighing between 180 g and 220 g, were randomly assigned to one of three groups: the control group (Con), the lipopolysaccharide (LPS) group, and the resveratrol (RSV) group. The resveratrol group received an intravenous administration of resveratrol at a dosage of 8 mg/kg, 10 min prior to lipopolysaccharide treatment. Each group comprised five rats. Various techniques including enzyme-linked immunosorbent assay (ELISA), hematoxylin and eosin staining (HE), periodic acid Schiff (PAS) staining, transmission electron microscopy (TEM), Western blot analysis (WB), and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to assess differences in inflammatory cytokine expression, histopathological changes, apoptosis, tight junction (TJ) protein, and the TLR4/MyD88/NF-кB signaling pathways. Resveratrol exhibited anti-inflammatory effects by decreasing levels of interleukin (IL)-1β, interleukin(IL)-6, and tumor necrosis factor (TNF)-α, while increasing interleukin (IL)-10. Additionally, in rats treated with resveratrol, there was a reduction in the expression of apoptosis-associated proteins Bax and Caspase-3. Resveratrol also significantly increased the expression of intestinal tight junction proteins (TJ), and decreased the levels of intestinal fatty acid binding protein (I-FABP) and D-lactic acid (D-LA). Furthermore, the expression of proteins in the related signaling pathways TLR4, MyD88, and NF-κB was decreased. Resveratrol has been shown to reduce the expression of intestinal apoptotic proteins, enhance the expression of intestinal tight junction proteins, and inhibit the inflammatory response mediated by the TLR4/MyD88/NF-κB signaling pathway, thereby alleviating LPS-induced septic intestinal injury.
肠道屏障功能是人体重要的防御机制，在脓毒症病例中既是主要靶点又是起始器官。维持该屏障的完整性对于预防包括脓毒症和死亡在内的并发症及疾病至关重要。尽管其重要性，但白藜芦醇对肠道屏障功能的影响仍不清楚。因此，本研究旨在探讨白藜芦醇对维持肠道屏障功能的潜在有益作用。将15只体重在180克至220克之间的雄性斯普拉格-道利大鼠随机分为三组之一：对照组（Con）、脂多糖（LPS）组和白藜芦醇（RSV）组。白藜芦醇组在脂多糖治疗前10分钟以8毫克/千克的剂量静脉注射白藜芦醇。每组包括5只大鼠。采用多种技术评估炎症细胞因子表达、组织病理学变化、细胞凋亡、紧密连接（TJ）蛋白以及TLR4/MyD88/NF-кB信号通路的差异，这些技术包括酶联免疫吸附测定（ELISA）、苏木精和伊红染色（HE）、过碘酸希夫（PAS）染色、透射电子显微镜（TEM）、蛋白质免疫印迹分析（WB）以及定量实时聚合酶链反应（qRT-PCR）。白藜芦醇通过降低白细胞介素（IL）-1β、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α水平，同时增加白细胞介素（IL）-10来发挥抗炎作用。此外，在用白藜芦醇治疗的大鼠中，凋亡相关蛋白Bax和半胱天冬酶-3的表达降低。白藜芦醇还显著增加肠道紧密连接蛋白（TJ）的表达，并降低肠道脂肪酸结合蛋白（I-FABP）和D-乳酸（D-LA）的水平。此外，相关信号通路TLR4、MyD88和NF-κB中的蛋白表达降低。已证明白藜芦醇可降低肠道凋亡蛋白的表达，增强肠道紧密连接蛋白的表达，并抑制由TLR4/MyD88/NF-κB信号通路介导的炎症反应，从而减轻LPS诱导的脓毒症性肠损伤。
