Totani Masayasu, Shinchi Hiroyuki, Kadokawa Jun-Ichi
Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan.
Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan.
Carbohydr Res. 2025 Mar;549:109373. doi: 10.1016/j.carres.2024.109373. Epub 2024 Dec 30.
We previously prepared self-reinforced chitin composite (SR-ChC) films, also called all-chitin composites, comprising two components, that is, scale-down chitin nanofibers (SD-ChNFs) with high crystallinity and scale-down low-crystalline chitin (SD-LC-Ch) matrixes. In this study, we precisely evaluated hydrophilicity under water enviromental conditions and its effect on cell adhesion using human-derived cancer cells on the SR-ChC film surfaces. The surface analysis of the SR-ChC films with reduced crystallinity revealed reorientation of the molecular chain assemblies with amino groups in the SD-LC-Ch components in water. Consequently, the amount of the SD-LC-Ch components gave rise to increasing the number of hydrophilic amino groups on the film surfaces, which resulted in efficiency of cell adhesion and elongation. This study concludes that the hydrophilic SD-LC-Ch components in the lower crystalline SR-ChC films strongly contribute to exhibiting new functions, related to interaction with biological substrates, such as cells. © 2017 ElsevierInc.Allrightsreserved.
我们之前制备了自增强几丁质复合材料（SR-ChC）薄膜，也称为全几丁质复合材料，它由两个组分组成，即具有高结晶度的尺寸缩小的几丁质纳米纤维（SD-ChNFs）和尺寸缩小的低结晶度几丁质（SD-LC-Ch）基质。在本研究中，我们精确评估了水环境条件下的亲水性及其对人源癌细胞在SR-ChC薄膜表面上细胞黏附的影响。对结晶度降低的SR-ChC薄膜进行表面分析发现，在水中SD-LC-Ch组分中带有氨基基团分子链组装体发生了重新取向。因此，SD-LC-Ch组分的含量增加了薄膜表面亲水性氨基基团的数量，从而提高了细胞黏附和伸长的效率。本研究得出结论，较低结晶度的SR-ChC薄膜中亲水性的SD-LC-Ch组分对展现与生物底物（如细胞）相互作用相关的新功能有重要贡献。© 2017爱思唯尔公司。保留所有权利。
