Prokofeva Maria I, Elcheninov Alexander G, Klyukina Alexandra A, Novikov Andrei A, Kachmazov Gennady S, Toshchakov Stepan V, Frolov Evgenii N, Podosokorskaya Olga A
Federal Research Center of Biotechnology, Winogradsky Institute of Microbiology, Russian Academy of Sciences, 60-let Oktyabrya prospect, 7, bld. 2, Moscow, Russia, 117312.
Gubkin Russian State University of Oil and Gas, Leninsky Prospect 65/1, Moscow, Russia, 119991.
Curr Microbiol. 2025 Jan 5;82(2):71. doi: 10.1007/s00284-024-04046-4.
A novel Gram-negative, motile, rod-shaped bacterium, designated 4137-cl, was isolated from a thermal spring of North Ossetia (Russian Federation). Strain 4137-cl grew at 30-50 °C (optimum 42 °C) with 0-3.5% NaCl (optimum 0-0.3%) and within pH range 4.0-8.7 (optimum pH 6.8-7.3). It was a strictly anaerobic microorganism capable of fermentation and respiration on organic acids and proteinaceous substrates. Sulfur, sulfite, polysulfide, and arsenate were used as electron acceptors. In addition to heterotrophic growth it grew autotrophically with H/CO. The major fatty acids were C ω8c and C. The size of the genome and genomic DNA G+C content of strain 4137-cl were 4.5 Mb and 59.2%, respectively. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 4137-cl represented a distinct lineage of the family Acetonemataceae within the class Negativicutes. As inferred from the morphology, physiology, chemotaxonomical and phylogenomic analyses, strain 4137-cl ought to be recognized as a novel genus for which the name Anaeroselena agilis gen. nov., sp. nov., we propose. The type strain is 4137-cl(=KCTC 25383 = VKM B-3575).
从北奥塞梯（俄罗斯联邦）的一处温泉中分离出了一种新型革兰氏阴性、具运动性的杆状细菌，命名为4137-cl。菌株4137-cl在30-50°C（最适温度42°C）、0-3.5% NaCl（最适浓度0-0.3%）以及pH值4.0-8.7（最适pH值6.8-7.3）的条件下生长。它是一种严格厌氧的微生物，能够在有机酸和蛋白质底物上进行发酵和呼吸作用。硫、亚硫酸盐、多硫化物和砷酸盐被用作电子受体。除了异养生长外，它还能利用H/CO进行自养生长。主要脂肪酸为C ω8c和C。菌株4137-cl的基因组大小和基因组DNA G+C含量分别为4.5 Mb和59.2%。根据16S rRNA基因序列和保守蛋白质序列系统发育分析，菌株4137-cl代表了Negativicutes纲内丙酮杆菌科的一个独特谱系。从形态学、生理学、化学分类学和系统基因组学分析推断，菌株4137-cl应被认定为一个新属，我们提议将其命名为敏捷厌氧硒菌属（Anaeroselena agilis），新属，新种。模式菌株为4137-cl(=KCTC 25383 = VKM B-3575)。
