Wang Xiao-Qian, Fan A-Qiang, Hong Liu
Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Air Force Medical University, Xi'an, 710032, China.
Sci Rep. 2025 Jan 6;15(1):871. doi: 10.1038/s41598-025-85321-7.
This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and examined the effects of its overexpression and knockdown on cell proliferation. Proteomic analysis was conducted to explore the interaction between MIR210HG and ferroptosis pathway components. The binding of MIR210HG to poly(rC) binding protein 1 (PCBP1) was predicted using catRAPID and confirmed through RNA pull-down and RNA immunoprecipitation (RIP) experiments. MIR210HG was significantly upregulated in colon cancer cells and tissues. Its overexpression promoted, while its knockdown inhibited, colon cancer cell proliferation. MIR210HG was found to be associated with ferroptosis pathway components and to bind to PCBP1, which was experimentally validated. The inhibition of ferroptosis by MIR210HG through PCBP1 binding was confirmed, highlighting its role in promoting cell proliferation. MIR210HG promotes colon cancer cell proliferation by binding to PCBP1 and inhibiting ferroptosis. These findings suggest MIR210HG as a potential therapeutic target for colon cancer.
本研究旨在探讨长链非编码RNA（lncRNA）MIR210宿主基因（MIR210HG）在结肠癌细胞增殖中的作用及其涉及铁死亡途径的潜在机制。我们评估了MIR210HG在结肠癌细胞系和组织中的表达，并检测了其过表达和敲低对细胞增殖的影响。进行蛋白质组学分析以探索MIR210HG与铁死亡途径成分之间的相互作用。使用catRAPID预测MIR210HG与聚（rC）结合蛋白1（PCBP1）的结合，并通过RNA下拉和RNA免疫沉淀（RIP）实验进行确认。MIR210HG在结肠癌细胞和组织中显著上调。其过表达促进结肠癌细胞增殖，而敲低则抑制增殖。发现MIR210HG与铁死亡途径成分相关并与PCBP1结合，这在实验中得到了验证。通过PCBP1结合，MIR210HG对铁死亡的抑制作用得到证实，突出了其在促进细胞增殖中的作用。MIR210HG通过与PCBP1结合并抑制铁死亡来促进结肠癌细胞增殖。这些发现表明MIR210HG是结肠癌潜在的治疗靶点。
