Huang Jianjun, Sha Ruyi, Dai Jing, Wang Zhenzhen, Cai Min, Li Xianxiu, Mao Jianwei
School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Food Chem X. 2024 Dec 9;25:102080. doi: 10.1016/j.fochx.2024.102080. eCollection 2025 Jan.
The potential applications of Bunge seed oil in the food and medical industries are constrained by its susceptible fatty acid composition, which is prone to oxidation. In this study, rice bran protein (RBP) was employed as an emulsifier for the fabrication of Bunge seed oil Pickering emulsion. The impact of antioxidant-phytic acid (PA) on the stability of Pickering emulsion and the underlying mechanisms were further investigated. The findings indicate that PA is capable of interacting with RBP, resulting in a change in its spatial conformation. When the PA concentration was increased from 0 to 0.01 % (/), the number of α-helices of RBP-PA particles decreased by 5 %, the number of β-sheets and interfacial adsorbed proteins increased by 2.89 % and 39.83 %. Additionally, and the surface hydrophobicity was increased from 50 ± 3.63 (a.u.) to 429 ± 20.03 (a.u.), and the range of the particle size distribution was reduced from 1 to 10 μm to 295-459 nm, and the zeta potential decreased from -23.43 ± 0.46 mV to -53.4 ± 1.35 mV. The Bunge seed oil Pickering emulsion, containing 0.01 % PA, exhibits favourable static stability and lipid oxidative stability, allowing for storage at room temperature for a period exceeding 50 days.
豇豆籽油易氧化的脂肪酸组成限制了其在食品和医药行业的潜在应用。本研究中,米糠蛋白(RBP)被用作乳化剂来制备豇豆籽油皮克林乳液。进一步研究了抗氧化剂植酸(PA)对皮克林乳液稳定性的影响及其潜在机制。研究结果表明,PA能够与RBP相互作用,导致其空间构象发生变化。当PA浓度从0增加到0.01%(/)时,RBP-PA颗粒的α-螺旋数量减少了5%,β-折叠和界面吸附蛋白的数量分别增加了2.89%和39.83%。此外,表面疏水性从50±3.63(a.u.)增加到429±20.03(a.u.),粒径分布范围从1至10μm减小到295 - 459nm,ζ电位从-23.43±0.46mV降低到-53.4±1.35mV。含有0.01%PA的豇豆籽油皮克林乳液具有良好的静态稳定性和脂质氧化稳定性,可在室温下储存超过50天。