Yang Cheng-Tse, Weng Pei-Wei, Chien Li-Hui, Kumar Sunil, Yang Ming-Ta
Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
Department of Orthopaedics, School of Medicine, Taipei Medical University, Taipei, Taiwan.
Biol Sport. 2025 Jan;42(1):137-144. doi: 10.5114/biolsport.2025.139087. Epub 2024 Jul 31.
Vitamin D exhibits strong antioxidant properties. However, its effect on oxidative stress after strenuous endurance exercise remains unclear. Therefore, we investigated the effects of vitamin D supplementation on strenuous endurance-exercise-induced oxidative stress. In this study, 26 male participants consumed vitamin D (5,000 IUs of vitamin D daily for 4 weeks) or placebo. After four weeks, all participants performed strenuous endurance exercise at 65% of O. Blood samples were collected before and after four weeks of supplementation to determine the participants' serum 25(OH)D concentration. Additionally, venous blood samples were collected before, immediately after, two hours after, and 24 hours after the strenuous endurance exercise test to analyze markers of oxidative damage (thiobarbituric acid reactive substances, and protein carbonylation) and antioxidant enzyme activity (catalase, glutathione peroxidase, and superoxide dismutase). After four weeks of vitamin D supplementation, the concentrations of protein carbonylation and superoxide dismutase at post-0-h, post-2-h, and post-24-h were significantly higher in the placebo group than in the vitamin D group (all p < 0.05); the concentration of thiobarbituric acid reactive substances was significantly higher in the placebo group than in the vitamin D group at post-24-h (p < 0.05); and the concentration of catalase was significantly higher in the placebo group than in the vitamin D group at post-0-h (p < 0.05). Our results indicated that four weeks of 5,000-IU vitamin D supplementation (oil form) effectively increased the participants' serum 25(OH)D concentration and mitigated the accumulation of strenuous-endurance-exercise-induced markers of oxidative stress (e.g., thiobarbituric acid reactive substances and protein carbonylation).
维生素D具有强大的抗氧化特性。然而，其对剧烈耐力运动后氧化应激的影响仍不清楚。因此，我们研究了补充维生素D对剧烈耐力运动诱导的氧化应激的影响。在本研究中，26名男性参与者服用维生素D（每天5000国际单位维生素D，持续4周）或安慰剂。四周后，所有参与者以65%的最大摄氧量进行剧烈耐力运动。在补充四周前后采集血样，以测定参与者的血清25(OH)D浓度。此外，在剧烈耐力运动测试前、运动结束后即刻、运动后两小时和运动后24小时采集静脉血样，以分析氧化损伤标志物（硫代巴比妥酸反应性物质和蛋白质羰基化）和抗氧化酶活性（过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶）。补充维生素D四周后，安慰剂组在运动后0小时、运动后2小时和运动后24小时的蛋白质羰基化和超氧化物歧化酶浓度显著高于维生素D组（所有p<0.05）；安慰剂组在运动后24小时的硫代巴比妥酸反应性物质浓度显著高于维生素D组（p<0.05）；安慰剂组在运动后0小时的过氧化氢酶浓度显著高于维生素D组（p<0.05）。我们的结果表明，四周补充5000国际单位维生素D（油剂形式）可有效提高参与者的血清25(OH)D浓度，并减轻剧烈耐力运动诱导的氧化应激标志物（如硫代巴比妥酸反应性物质和蛋白质羰基化）的积累。
