Sousa Ana, Chambion-Diaz Marie, Pialoux Vincent, Carin Romain, Viana João Luís, Milheiro Jaime, Reis Víctor Machado, Millet Grégoire
Research Center in Sports Sciences, Health Sciences and Human Development - CIDESD, University of Maia, UMaia, Maia, Portugal.
Laboratoire Interuniversitaire de Biologie de la Motricité, Univ Lyon, University Claude Bernard Lyon 1, Villeurbanne, France.
Biol Sport. 2025 Jan;42(1):243-251. doi: 10.5114/biolsport.2025.139851. Epub 2024 Nov 18.
Oxidative stress is augmented under hypoxic environments, which may be attenuated with antioxidant supplementation. We investigated the effects of dietary nitrate (NO-) supplementation combined with high-intensity training performed under hypoxic conditions on antioxidant/pro-oxidant balance. Thirty trained participants were assigned to one of three groups - HNO: hypoxia (13% FO) + NO-; HPL: hypoxia + placebo; CON: normoxia (20.9% FO) + placebo - while performing 12 cycling high-intensity interval training (HIIT) sessions during a 4-week period (3 sessions/week). Before and after the intervention, venous blood samples were collected and a time to exhaustion test (T) was performed (with vastus lateralis changes in local O saturation: SmO monitoring). Glutathione peroxidase (GPX) activity increased in CON (p = 0.017, ~20%) and superoxide dismutase (SOD), catalase and ferric-reducing antioxidant power (FRAP) did not change in any group. Malondialdehydes (MDA) increased in both HNO (p = 0.001, ~60%) and CON (p = 0.023, ~30%) but not in HPL. Advanced oxidation protein products (AOPP), uric acid, and myeloperoxidase activity were not modified by the protocol. Only the CON group recovered faster after the T test (SmO: p = 0.0003, ~58%). Hypoxic exposure during high-intensity training blunted the increase in GPX and MDA after the intervention period. However, the effects of NO- supplementation seem to very slightly mitigate the detrimental effect of performing high-intensity training under hypoxic conditions.
在缺氧环境下氧化应激会增强，而补充抗氧化剂可能会减弱这种应激。我们研究了在缺氧条件下进行高强度训练时补充膳食硝酸盐（NO-）对抗氧化/促氧化平衡的影响。30名受过训练的参与者被分为三组之一——HNO组：缺氧（13%氧气）+NO-；HPL组：缺氧+安慰剂；CON组：常氧（20.9%氧气）+安慰剂——在4周内进行12次自行车高强度间歇训练（HIIT）课程（每周3次）。在干预前后，采集静脉血样本并进行力竭时间测试（T）（同时监测股外侧肌局部氧饱和度变化：SmO）。CON组谷胱甘肽过氧化物酶（GPX）活性增加（p = 0.017，约20%），而超氧化物歧化酶（SOD）、过氧化氢酶和铁还原抗氧化能力（FRAP）在任何组中均未改变。丙二醛（MDA）在HNO组（p = 0.001，约60%）和CON组（p = 0.023，约30%）中均增加，但在HPL组中未增加。高级氧化蛋白产物（AOPP）、尿酸和髓过氧化物酶活性未因该方案而改变。只有CON组在T测试后恢复得更快（SmO：p = 0.0003，约58%）。高强度训练期间的缺氧暴露使干预期后GPX和MDA的增加减弱。然而，补充NO-的效果似乎非常轻微地减轻了在缺氧条件下进行高强度训练的有害影响。
